| In this study, the transgenic anti-insect hybrid cottons varieties (including Nong Za-62F1, Nong Za-62F2, Nong Za-62 F3, H16F1, H16F2, H16F3, H64F1, H64F2, H64F3) and non-transgenic cotton (No.SiMian3) that is the check variety were investigated to compare F1, F2, and F3. The main results were as following:1. The developmental period and rate of hybrid cottons in FI were similar to that of in F2 and F3. Generally the tendency of plant height, the number of fruit branches and setting bolls were F1> F2> F3>CK. There were no significant difference on plant height and number of fruit branch between F1 and its offspring F2 and F3, but there were significant differences on total of mature bolls between F1 and its offspring F2 and F3. Mean of bolls per plant in F1, F2, F3 was 33.7, 31.2 , and 30.6, respectively. F1 had more 2.5, 3.1 bolls compared with F2, F3, respectively. The percentage of pre-hot summer bolls and hot summer bolls in FI was higher than that in F2 and F3, The percentage of pre-hot summer bolls and hot summer bolls in F1, F2, F3 were 66.07%, 61.69% and 61.54 %, respectively. The percentage in F2 was less 4.38% than that in F, and was similar to that in F3. The general tendency of the percentage of number flowers before frost stage to the total flowers was F1> F2 >CK> F3. The highest percentage of Nong Za-62F1 was 69.09%. The mean of percentage of F1, F2 and F3 were 66.06%, 62.37%, and 60.07%, respectively. The percentage of F1 was higher 4.75% than CK.2. Physiological researching in different generations of hybrid cotton showed that: (1) the chlorophyll content in leaves varied with time, and was higher at early growing stage and was lower at late growing stage. The nitrogen metabolize in leaves at early stage was stronger than that at late stage. The nitrogen metabolize decreased with passing time; (2) the yields in different generation was accordance with photosynthesis accumulation at boiling stage. The general tendency of chlorophyll content and nitrogen metabolize was F1> F2> F3>CK at the same stage. The chlorophyll a content of F1 had more 0.33μg/mK 0.77μg/ml and 1.44μg/ml compared with F2, F3,CK , respectively at primary blossom phase. The chlorophyll b content of FI had more 0.70(0, g/ml> 0.75μg/ml> and 1.83μ g/ml compared with F2, F3,CK, respectively at primary blossom phase. The chlorophyll a content of FI had more 0.99μg/mK 1 01μ g/ml and 1.44μg/ml compared with F2, F3,CK, respectively at primary opening boll. The chlorophyll b content of FI had more 0.46μg/mK 0.75μg/ml and 1.43μg/ml compared with F2, F3,CK , respectively at primary opening boll. The nitrogen content of F1 in leaves was higher 0.13%, 0.20% and 0.28% compared with F2, F3,CK, respectively at primary blossom phase, but there were no significant difference at primary opening boll.3. The resistance to insect in F2, F3 separated obviously. All plants in F1 selected by Knanmycin sulfate had resistance to insect, but about 20% plants in F2, F3 selected by Knanmycin sulfate showed no resistance. The percentage of damaged apexes of stem and squares and bolls in the field showed that the resistance to target insect in F2 at early growing stage was similar to that in FI, and decreased at late stage, the percentage of damaged apexes and squares and bolls increased obviously. The average percentage of boll shedding of F1, F2, F3 and CK were 6.21%, 13.05%, 17.91% and 29.63% respectively. And the percentage of boll shedding of F1, F2, F3 reduced by 23.423%, 16.58%, and 11.72%, respectively compared with CK. This was to say planting anti-insect hybrid cotton might increased yields.4, Yield analysis showed that average yield of F1 was 1296.30kg/ha, and increase by 282.45kg/ha compared with CK and its average competitive dominancy was 27.86%; Average yield of F2 was 1168.65kg/ha, increased by 154.80kg/ha compared with CK and its average competitive domain was 15.27%; Average yield of F3 was 1117.65kg/ha, increased 103.80kg/ha compared with CK and its average competitive dominancy was 10.24%. If F1 had higher competitiv...
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