Systemic Reasearch On Laser-Mutagenesis Of Seedless Shatian Pumelo

Shatian Pumelo is a kind of high quality fruit in the world market. Our group of experiment have got the seedless fruit by the means of Laser-mutagenesis.Most experimental swatches and data are included in this paper. Many experiments are done and analyzed in two aspects of biological and physical.From biological aspect, we observed the modality aberrance, discussed the connection between pollen abortion and Laser-mutagenesis of seedless Shatian Pumelo in morphologic, cytology, genetics and so on. We observed and analyzed the male and female embryology's development, enzymology, chromosome structure and number, gene et.cl.From theory analysis aspect, we analyzed the theoral mode of aberrance DNA acted by Laser. The main quanta count was cited from Rein's DNA results. We disposed the data by the means of Curve Least Square, made out proximate equation, and then calculated DNA H-bond system proton trans-coefficient. The result can be used to parse Laser'sact infection on DNA mutation rate. The mutation mechanism wasexplained at the part of theory.The following is our results and conclusions:1. The biological experimental result: the contrast between experiment and wildness Shatian Pumelo is that the male and female embryology's development is normal, enzymologist work character, pollen abortion and DNA are different. The result shows that laser can lead species mutation.2. Multiplex Laser makes enzymologist chart super positional. Enzymologist chart has changed, and the second generation's seedless character is steady on the whole. The result shows that the inherited character by Laser-mutagenesis is possible.3. Shatian Pumelo's ovule impregnated through pollen pollination, and then developed into seed in fruit. If abortion appeard on pollen after Laser- irradiation, the number of seed can be reduced greatly. It's evident that the seedless is connected with pollen abortion. But it is not sure that pollen abortion can bring seedless absolutely. Inter reasonal pollen abortion can bring seedless fruit. So pollen abortion is possible only one of reasons that bring seedless fruit.4. The Laser-mutagenesis seedless fruit's burgeon leafage DNA sequence is changed. Contrast with the weedless, it has genetic difference. So it grows into seedless fruit.5. Seedless Shatian Pomelo's fruity ratio is lower, the seed capsule is thicker. But we have got the seedless.character.6. The main mechanism that low-power Laser affected on organism and got variation is Laser phonic and electromagnetic effect. Any wavelength laser can bring biological effect.7. Species variation ratio is very little in nature condition, the ratio can be rised by Laser but still little. So we should choose laser bean parameter in order to avoid blindness work.