| 12 kinds of fungi were got by separating, culturing and purifying from sporocarps which were gathered in the mixed forest of red Picea koraiensis. 6 kinds of ectomycorrhizal fungi were got by manual inoculation to Picea koraiensis seedlings in the laboratory which are strain 004. Agaricus silvaticus, strain L15, strain 025, strain 009 and strain 031.Biological mensuration indoor indicated that photosynthesis of seedlings which form mycorrhiza were enhanced, content of chlorophyll were augmented, root were prosperous, weight and height were increasing obviously. To content of chlorophyll, the seedlings inoculated Agaricus silvaticus increase 32.57%, which inoculated strain LI 5 increase 31.92 %. To weights seedlings inoculated 6 stains enhance 9.62 %~ 23.08%. The height of seedlings inoculated 6 stains increase 4.00%~17.84%.Inoculation test outdoor showed that Agaricus silvaticus is the best to seedlings height growing, strain L1 5 is the best to seedlings diameter growing, strain 009 is the best to root growing, and to infection rate and photosynthesis strain 031 is the best. To sum up, strain 031, strain 009 and strain L1 5 should be fine strains to seedlings of Picea koraiensis. By biological characteristic test the optimum growth temperature, optimum carbon source, optimum nitrogen source and optimum pH of 6 ectomycorrhizal fungi are determined. The optimum growth carbon source of strain 004 is diabetin, that of strain L1 5 and 031 is glucrose, that of Agaricus silvaticus is sucrose, strain 009 and 025 is maltose. Optimum growth nitrogen source of Agaricus silvaticus and strain 031 is Asparagine, that of strain 009 is NaNO3, that of strain 025 is ammonium tatrate, and that of strain 004 is (NH4 )H2PO4. The temperature of optimum growth of strain 004 and 009 is 20℃, that of strain 031 and Agaricus silvaticus is 25℃ that of strain 025 and LI 5 is 30℃. Except strain 025, the optimum growth pH of other 5 strains is 6.5.
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