| It regarded stem tip of Tillered-onion as explants,on the basis of the technical exploration of tissue culture in Tillered-onion,it adopted the pathway which induced test-tube seedlings from explants then induced bulblets,after this,it explored the technique of micropropagation which proceeded from the condition of tissue culture,in order to solve the malady in traditional technique of interface,and provided factory production of bulblets from virus-free seedlings,the conclusions were as follows:1 the technique of tissue culture for test-tube seedlings(1)The mediums of MS+NAA0.1mg/L+BA0.7mg/ L and MS+NAA0.1mg/L+2iP0.7mg/L were both fit for the culture of induction.(2)The mediums of MS+IBA2.5mg/L and 1/2MS+IBA1.5mg/L +NAA0.05mg/L were both fit for the culture of rooting.(3)Raising the rate of C/N was advantageous for the culture of rooting.(4)The relative lower concentration of IBA or NAA were advantageous for the culture of rooting,and the effect of IBA was superior to NAA.2 The micropropagation technique of test-tube seedlings(1)The source of carbohydrateAll the test-tube seedlings could grow normally on the mediums which regarded sucrose(chemical purity and analytical purity),glucose or soft white sugar as the source of carbohydrate,fructose was not fit for growth,soluble starch could not be served as the source of carbohydrate；The test tube seedlings could become bulblets on the mediums which regarded sucrose(chemical purity and analytical purity),glucose ,fructose or soft white sugar as the source of carbohydrate,and sucrose of analytical purity was the best one,which the No. and the fresh weight of bulblets came up to 39.67 and 64.93mg respectively,The second one was sucrose of chemical purity,soft white sugar was in the middle,glucose and fructose were the poorest,soft white sugar was a good source of carbohydrate,for growth and bulblet formation,it could become the substitute of sucrose.(2)The concentration of sucroseThe higher concentration of sucrose could inhibit the growth of test-tube seedlings,the seedlings were difficult to form leaves and roots,and were difficult to absorb water,too；Within a certain range,raising the concentration of sucrose could promote bulblets,and the bulblets could form earlier when raising the concentration.But the excessive high concentration(150g/L)could weaken the effect；120g/Lwas the optimal concentration for bulblets,the mean No. and the rate of bulblets came up to 31.33 and 90.00% respectively.(3)PhytohormonesIt was advantageous for bulblet formation when BA and (or)NAA were lower or without phytohormones；NAA1.0mg/L was the best one for the culture of bulblets,the diameter and th fresh weight of bulblets came up to 4.02mm and 115.73mg respectively.(4)PP333Within a certain range,raising concentration was advantageous for bulblet formation,but the excessive high concentration(10.0mg/L)could weaken the effect；The medium of 5.0mg/L PP333 was the optimal one,the mean No.,rate,diameter,fresh weight and index of bulblets came up to 41.00,92.86%,11.55mm,709.06mg and 1.10；PP333 had powerful effect of dumpiness and inhibition, within a certain range,the effect became stronger when raising concentration,the index<1 could be found,and the bulblets became flat round from ellipse gradually ,PP333 could postpone growth,the process of bulblet formation was over 150d；The bulblets on PP333 were light green,and the purplish red peels were relative fewer.(5)S-3307Within a certain range,raising concentration was advantageous for bulblet formation；1.0mg/L S-3307 was the optimal one,the mean No.,rate,diameter,and index of bulblets came up to 45.67,93.34%,8.52mm and 1.21；S-3307 had powerful effect of dumpniss,within a certain range,the effect became stronger when raising concentration,the index<1 could be found,and the bulblets became round from ellipse gradually；S-3307 could postpone growth,the process of formation for bulblets was over 150～210d；The bulblets on...
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