Study On The Main Influencing Factors Of Tillering Onion Stem Plate Tissue Culture

Tillering onion(Allium.cepa L.var.agrogatum Don.)is an important vegetable crop,which is mainly distributed in Jilin,Heilongjiang and other regions.Bulbs are used as asexual propagation material to reproduce vegetatively throμghout the year,causing serious disease infections and affecting industrial development.The use of tissue culture technology to form test tube bulbs can improve this situation,but the growth cycle of test tube bulbs is too long,the reproduction coefficient is not high,and it is difficult to achieve factory cultivation.In this experiment,the stem disk of tillering onion was used as the experimental material,and the tissue culture technique was used to screen the explants of germinating bulb and growing plant bulb of tillering onion,and different inoculation lengths of explants were set.In order to obtain the primary tissue culture seedlings with high germination rate and strong seedling growth.On this basis,the experiment of subculture propagation of primary tissue culture seedlings was carried out,and the split zone design of two factors was adopted.Firstly,the effects of different growth height and different longitudinal cutting ratio of primary tissue culture seedlings on the growth of subculture seedlings were compared.After that,the culture conditions of subculture seedlings were further optimized.Throμgh the experimental study,it is concluded that the germination rate and rooting rate of the tissue culture seedlings obtained from the germinating bulbs of tillering onions as explants are better than those obtained from the bulbs culture of growing onions,and the best inoculation length of explants is 12 mm.In the propagation experiment of tissue culture seedlings,the growth height of primary tissue culture seedlings as propagation material was the best with 60 mm,and the best proportion of longitudinal cutting was 1 cut 2.In the experiment of optimizing the culture conditions of propagated seedlings,the best environmental conditions for the growth of propagated seedlings were 2000 Lux.light and 25 ℃.Tissue culture technique was used to disinfect the shoot tip of tillering onion in vitro,and a large number of tissue culture seedlings were obtained by primary culture and subculture.In the next step,the rapid propagation of tillering onion can be realized by direct transplanting after seedling refinement.The subculture propagation of tissue culture seedlings can greatly reduce the growth time of tissue culture seedlings in the laboratory and expand the propagation coefficient at the same time.