Inheritance And Molecular Marker Analysis Of Sharp Eyespot Resistant Gene In Wheat

In this study, RIL populations are used to analyse the inheritance of sharp eyespot resisitant gene in wheat. The linkage map of SSR and ISSR molecular markers is completed using RIL-8 population. The linkage-ship between molecular markers and sharp eyespot resistant gene was analysed using RIL-SES population.The main results are as follows.15 RIL populations are used to analyse inheritance of sharp eyespot resistant gene. The resistant gene is controlled by major genes and mutipple genes. When two major genes exist, they always exsist epistatic effects which values are generally smaller than additive effects. Contribution of major gene is greater than mutipple gene, so major gene is more important. Based on the RIL-8 population, a genetic map including 42 SSR locis was constructed. Adding Li'job, a genetic map including 94 SSR locis, 31 ISSR locis and 3 HMW-GS locis distributing 20 chromosomes (not including 3 A) was constructed.Based on the genetic map with 128 distributed molecular marker locis, we indentified 1 addative and 4 pairs of epistatic quantitative trait loci(QTLs) contributing to sharp eyespot resistance. The addative QTL locating 1A chromosome could explain 21.57% of the total phenotypic variation. 4 pairs of epistatic QTLs which interaction effect values were 11.63%,6.54%,14.04%,20.01%, locate on 7 chromosomes , and their total interaction effect value was 52.23%.RIL-SES population with 118 individuals was used to indentify molecular markers linked to sharp eyespot resistant gene. As a result, 2 molecular markers, including 1 SSR and 1 ISSR marker, were detected linked to sharp eyespot resistant gene. This dominant resistant gene was mapped on chromosome 2B. The linkage distance between the markers was 27.9cM and 16.9cM.