AFLP Characterization Of Carposina Sasakii Complex

The peach fruit borer, Carposina sasakii Matsumura complex, is an important fruit pest in China, Korea, and Japan. It can cause severe damage on many kinds of fruit trees, such as apple, peach, apricot, common jujube, hawthorn, wild jujube.In this study, seven different host races infesting various fruit trees including apple, peach, apricot, common jujube, hawthorn, wild jujube, Malus asiatica var. rinki and one out group dogwood, were used to study the genetic distance and relationship of C. sasakii complex by AFLP technique (Amplified Fragment Length Polymorphism). Procedure for C. sasakii AFLP analysis is established.The genomic DNA from the eight host races were extracted by means of improved CTAB respectively. The genomic DNA pool was composed of the mixture of the 8 samples from different host races with the same quantity. The provided DNA was suitable for AFLP analysis.TaqI/PstI was selected from five different combinations. The fingerprint displayed that the combination meeted with AFLP analysis of C. sasakii.Ten primer combinations with the good polymorphic amplified band were selected from 15 primer combination. Ten AFLP primer combinations generated 771 reliable AFLP bands, average 77.1 per primer combination, of which, 741 bands were polymorphic, revealing 96.1% of polymorphic bands in the total bands and the mean value was 74.1. The different host races had the different total bands by amplification of 10 primer combinations. Apricot host race had the most amplification bands, which was 437, average 43.7 per primer combination. M. asiatica var. rinki host race had the lest amplification bands, which was 250, average 25.0 per population.Genetic distances among eight different host races were analyzed using reliable AFLP bands by NTSYS-pc software. Genetic distances were calculated for constructing a dendrogram by UPGMA and Nj clustering analysis. Genetic relationship was revealed by the dendrogram. The genetic relationship was closest between peach and apple host races. Genetic relationship was closer between common jujube and apricot host races than other host races, however the genetic relationship among hawthorn, wild jujube, M. asiatica var. rinki and dogwood host races were far one by one.