Proteomic Of Lack-Lateral-Root Mutant Rice RM109

Plant roots serve important functions.The root of terrestrial plant are involved in the acquisition of water and nutrient in the soil, anchange of plant, synthesis and secretory of plant hormones and regulation of life activity .The development of a root system involves strategies that are common to the development of all plant organ.Lateral roots and root hair can increas root absorbing area directly. The amount and development of lateral roots also can affect the absorbing of water and nutrients, whatsmore, affect the product of food. So it is very important to study the mechanism of lateral root.Protein is the unit of biology structure and function. According to the central rules, it seems that there has a linear relation between gene and protein. However, it is not as much as people think. Recently, the level of protein and transfer in cell is lack of ralitivity. DNA and RNA are unable to reflect the expression, modification and reciprocity of protein correctly. Thus, to reveal the mystery of life, the aim of the proteomics is to study widly and cosmically.We analyse groups of mutants and their wild type, use a series of methods, including immobilized pH gradient two dimensional polyacrylamide gel electrophoresis, commassio staining, PDQuest 2-DE software, peptide mass fingerprinting based on MALDI TOF MS and swiss-PROT database searching and set up a proteomic approach. We emphasized to analyse proteomic of lack-lateral-root mutant and its wild type. Comparision of their maps, 40 different spots were digested in gel with TPCK-trypsin and measured with MALDI TOF MS, and 20 protein were identified, which may help to search mechanism of the development of lateral root.