Studies On The Effect Of Oyster Shell Soil Remediation On The Physiologic And Biochemical Activities Of Glehnia Littoralis

Oyster is a famous economic shellfish with high nutritive value.It's resource is abundant and yield is the largest in shellfish.Large quantities of oyster shell are abandoned, which not only pollute the environment ,but also waste resources. Oyster shell contains more than 90% calcium carbonate and kinds of trace elements and a few organic matter.Oyster shell has applied value in medicine, functional food,and sorts of additives.At present, the utilizing rate of oyster shell is low because studies on the comprehensive utilization of it is scare.In this paper, effects of soil remediation which basic ingredient is oyster shell on the physiologic and biochemical activities of Glehnia Littoralis are studied from several aspects.The objective of this study is to oifer theoretical evidence for the comprehensive utilization of oyster shell via analyzing the influence of soil remediation on plant.This study includes six parts: the effects of soil remediation on Glehnia Littorali/ absorptive capacity, chlorophyll content, main effective components content and yield, and the inducing effects of soil remediation on Glehnia Littorali^s defence enzyme activity and heat shock protein.Experiment of heat shock protein is accomplished in laboratory, the other experiments are accomplished by field test. Experimental materials are divided into four treatments: control, 375kg/hectare, 750kg/hectare and 1125kg/hectare.Studies on soil nutrient show that soil remediation promote the absorption of Glehnia Littoralis to soil nitrogen,phosphorus,and organic matter. Promotive effect on quick result nitrogen and phosphorus are most evident.Significance difference between 750kg/hectare treatment and control group are observed.Chlorophyll content is studied by spectrophotometric method. Results show that soil remediation can increase chlorophyll content,and the higher concentration of soil remediation add,the more effect is seen. There is a significance difference between 1125kg/hectare treatment group and control.Analysis of the yield , contents of polysaccharide, amino acid, extracts of water and alcohol of Glehnia Littoralis show that soil remediation can improve the yield of Glehnia Littoralis, and the yield increases with the higher concentration. But,when the concentration exceeds 750kg/hectare, the yield doesn't increase evidently. Soil remediation can also increase the content of polysaccharide, amino acid, extracts of water and alcohol of Glehnia Littoralis to some extent. Treatment with 375kg/hectare soil remediation doesn't show evident effect, 750kg/hectare and 1125kg/hectare are optimum concentration.Studies on four defence enzyme activities of Glehnia Littoralis show that soilremediation can elicite phenylalanine ammonialyase,peroxidase,chitinase and?-1,3-glucanase activities effectively and the levels of increase of chitinase and?-1,3-gluccanase activities are higher than that of phenylalanine ammonialyase and peroxidase.The effective concentrations are 750kg/hectare and 1125kg/hectare .Glehnia Littoralis seedling of four treatments are heat shocked at 43 癈 for 10 hours.The results show that concentrations of 750kg/hectare and 1125kg/hectare can elicite heat shock proteins' synthesis and 750kg/hectare is the best concentration.Above results indicate that soil remediation which basic ingredient is oyster shell can promote absorption of soil nutrient, improve chlorophyll content and effective component's content of Glehnia Littoralis. Soil remediation can also induce defence enzyme activity to increase and heat shock protein to synthesize.