Comparative Research On The Litter Size Relevant Gene Of Wild Boar Swine And Hybrid Pig

The litter size of pig is a low heritance quantitative trait(h2=0.1),in conventional methodto improve this trait almost have no effect.Since the 1980's, a set of scientist as well asRothschild act as deputation,through the method of genetic marker and marker assitedselection,had found some candidate genes about litter size trait. Because there is a most different between wild boar and pig in this trait,we wanted tothrough comparing the base sequence of wild boar and pig in these gene which had beenconsidered as the candidate gene-ESR,FSH?,LH,PRLR and RYR1.The deputation of pigis native pig breeds of Min Pig,introduced pig breeds of Landrace and developed pig breedsof double muscle pig.Using technology of PCR-SSCP. Result:there is not most different in base sequence in large PCR fragment,only havesome base different.But some different bases are locus on exon,they may lead to some crucialamino acid change and function change.We design primer in changed points to examinewhether these change are all in three pig, PCR-SSCP technology was applied to test.Result:There is one mutant site in the fragment of the second primer of ESR: A→G,it lead aminoacid change: Arg→His,both pig and wild boar are all have no BB genotype individual,but thefrequence of B gene of wild boar is more higher than pig's.There is two mutant site in thefragment of the first primer of FSHβ: C→T, G→A,it lead to amino acid change: Phe→Ser,Ala→Thr, they all have three genotype except shuangjitun has no AA.There is one mutant sitein the fragment of the third primer of FSHβ:C→T,no amino acid change,so I think there isno difference in three pig breeds.There is two mutant site in the fragment of the third primerof LH: T→G,C→T, no amino acid change,no difference.There is two mutant site in thefragment of the second primer of PRLR: C→T,no amino acid change, T→C it lead to aminoacid change: Val→Ala,the result of statistical analysis is hybrid pig have no BB,CC genotypeindividual,wild boar have no BB genotype,pig all have,but the individual of BB,CC arelittile,too.Taking one with another,it is different in this fragment of PRLR gene among thesepig breeds.There is one mutant site in the fragment of the third primer of RYR1: C→T, noamino acid change,no difference.Take into account whole,there is some difference in the fivegene of part sequence among wild boar,pig and hybrid pig. IIABSTRACT Master candidate:Zhang Dongjie Specialty:Animal Genetics and Breeding Supervisor:Liu Di...