Effect Of Vitamin E Levels On Immune Function Of Juvenile Jian Carp (Cyprinus Carpio Var. Jian)

Two expriments were conducted to study the effect of vitamin E levels on immune function of juvenile Jian Carp (Cyprinus carpio Var. Jian). In trial I , 264 juvenile Jian carps(inital weight 7.78 + 0. 02g) were randomly allocated to 2 groups feeding the semi-purified diets containing vitamin E 4 or 97mg. kg-1 respectively for 70 days. In trial II, 792 juvenile Jian carps(inital weight 7.78 + 0.02g) were randomly allocated to 6 groups feeding semi-purified diets containing vitamin E 97,490, 1487,2490,4825 and 9858mg. kg-1 respectively for 70 days.In trial I ,we found weight gain, feed intake, feed conversion efficiency, protein retention rate(PRR), intestinal folds s hight , hepatopancrease weight/body weight, vitamin E content in hepatopancrease, head kidney weight, head kidney weigh! /body weight, spleen weight. spleen weight /body weight, blood hematocyte and leukocyte counts, serum malondialdehyde Content, phago-rate of leukocyte, survival rate, serum lysozyme activity, serum antibody titre after vaccination against Aeromonas hydrophila decreased great significantly or significantly as juvenile Jian carps feeding vitamin E deficiency diet(P<0.01 or 0.05). Post kidney weight, post kidney weight/body weight, survival rate during experimentation were not observed significant differences between two treatment groups (P>0.05). These results indicate that Juvenile Jian carps appeared the resisting diseases s ability decreasing, with head kidney and spleen growth retardating, resisting oxidation ability, non-specific and specific immune ability decreasing as l'eeding vitamin E deficiency dietIn trial 11, indices, such as feed intake, weight gain, feed conversion efficiency, PRR, hepatopancrease weight, intestine weight, intestine length, intestine length/body weight, intestinal folds s, head kidney weight, spleen weight, head kidney weight/body weight, spleen weight/body weight, hepatopancrease weight/body weight, blood hematocyte counts, blood leukocyte counts, serum malondialdehyde Content, phago-rate of leukocyte, survival rate, serum lysozyme activity and serum antibody level at 17d after vaccination against Aeromonas hydrophi la were great significantly or significant differences among experimental groups (P<0. 01 or 0. 05). but another indices, such as post kidney weight, post kidney weight/body weight and intestine weight/body weight were not significant differences(P>0.05). There was significant quadraticly relationship between weight gain or PRR and logVE levels(R12=0. 7850,P<0.01; R22=0. 7260, P<0. 01). Signi ficant correlations between weight gain and PRR (r=+0. 9960, P<0. 01) . There was significant quadraticly relationship between scrum lysozyme activity and logVE (R2=0.7040, P<0.01). There was significant quadraticly relationship between survival rate after vaccination against Aeromonas hydrophila or post kidney weight or spleen weight or serum antibody level at 17d after vaccination and vitamin E levels (R12=0.7020, P<0.01; R22=0.6420, P<0. 01; R32=0. 5990, P<0. 01 ; P42=0.7210 , P<0. 01). Significant correlations between survival rate after vaccination against Aeromonas hydrophila and post kidney weight or spleen weight or serum lysozyme activity or 17d antibody level after vaccination existed(r1=+0.8780,P<0.05 ; r2=+0. 9090, P<0. 05; r3=+0. 8820, P<0. 05; r,=+0. 8490, P<0. 05). But weight gain, PRR, survival rate after vaccination against Aeromonas hydrophila, phago-rate of leukocyte, serum lysozyme activity and serum antibody level at 17d after vaccination against Aeromonas hydrophila indice decreased great significantly or significantly while fish feeding diets containing vitamin E 4825 and 9858mg. kg-1(P<0. 01 or 0. 05), feed conversion effieiency and serum malondialdehyde Content increased great significantly while fish feeding diets containing vitamin E 4825 and 9858mg.kg-1 (P<0. 01 or 0.05) . These results indicate that higher level vitamin E were able to increase growth and resisting diseases s ability of juvenile Jian carps by affecting head kidney growth, spleen weight, non-speci...