Studies On Tissue Culture And The Callus's UV-B Irradiation Of 'Red Globe' Grape (Visit Vinifera.'Red Globe')

In this study, stems and leaves of Red Globe 'grape (Visit vinifera. 'Red Globe' ) were cultured in vitro .Several factors affecting stems culture and callus inducaton from stems and leaves were studied. The suitable time of UV-B irradiation was investigated.The results showed:(1) Stem culture The base or the middle of the stem was more suitable for bud sprouting.(2) The suitable placement manner of stems for bud sprouting was the erect manner.(3) The optimum medium for the stem's initial culture was B5+BA2.0mg/L + KT1 .Omg/L,in which the germination rate could reach 85.7%.(4) The optimum medium for the stem's muitiplication was B5+BA2.0mg/L+IBA0.1mg/L,in which the multiplication rate could reach 5.82 and the sprout grew well.(5) The optimum medium for rooting was B5+ NA A 0.1 mg/L +0.1 %AC, in which therooting rate reached 100%(2) Callus induction from the stem and leaf and the UV-B irradiation experiment CD The optimum medium for inducing callus from the leaf was MS+6-BA1.5 mg/L +2 ,4-D1.5 mg/L, in which the inducing rate could reach 91.4%.(2) The optimum medium for multiplication of leaf's callus was MS+6-BA0.5 mg/L+ KT0.5 mg/L+2,4-D 1.5- 2.0 mg/L, in which the multiplication rate reached 2.0.(3) According to the death time of leaf's calluses ,the suitable UV-B irradiation time wasl-2min.(4) The optimum medium for inducing callus from the stem was B5+BA1.5mg/L +IBA0.3mg/L, in which inducing rate could reach 95.8%.(5) The optimum medium for multiplication of the stem's calluses was B5-+BA1.0mg/L+KT0.25mg/L+IBA0.1 mg/L +NAA0.1mg/L, in which the multiplication rate reached5.7.(6) According to the living rate and growth status of stem's calluses, the suitable UV-B irradiation time was 3-4min.