Studies On Grafting And Transplanting In Dodecaploid Plantlet Of Nonastringent Persimmon

Dodecaploid plantlet of nonastringent persimmon is important material of sexual breeding. However, it has not been exerted because abandance flock tannin hampers rooting and transplanting of plantlet. In vitro grafting of plantlet is of effective method which helps to resolve the problem of rooting and transplanting. There has not been reported about in vitro grafting of plantlet of nonastringent persimmon. In virtue of grafting of twig, dodecaploid plantlet of nonastringent persimmon was used as material to study in vitro grafting of plantlet. At the same time, plantlet transplanting was also studied and the major results were as follows:1. The optimum period of embryo culture of rootstock was during 60~90d after flowering in 'Zenjimaru' persimmon.2. The survival rate of grafting was highest that the seedling rootstock, was 20-30 d old, green and half-lignificated with cotyledon outspread while new leaf not sprouting.3. The optimum length of graft-cut was 2-3mm on the base of keeping full root and excising cotyledon.4. The optimum medium was solid MS (1/2N) supplemented with IB A 0.3 mg/L+ZT 0.44 mg/L-f AC 3 g/L.5. Grafted plant with the bud of rootstock being removed should be placed into the original medium. Otherwise, it woule result in a great lot of death if grafted plant was transferred to new medium or supplemented with new liquid nutriment.6. Survival rate of plantlet could be reached 80% if 'buttle-closed exercise' should have been for 3~4 weeks in the scattered light before plantlet was transplanted .