Isolation And Identification Of Pathogenic Bacteria Caused The Rot Disease In Ginger And The Filtration Of Suppression Compounds

Rot disease in ginger is a truculence disease to ginger planting. The reduction of output of the bad field can be up to 50 percent. The disease badly affects the pocketbook of ginger farmer. We isolated the pathogenic bacteria from the naturally affected ginger by dilute with grads, and identified them as Pseudomonas. solanacearum.It is controlled by medicament for long, but we still have not a specific medicine up to the present. We compared the inhibition of existing pesticides and antibiotics, used several medicaments to suppress them and find out the most two effectivecompounds-2,4,4'-trichloro-2'-hydroxy-diphenyl etherand 2,4,4'-trichloro-5'-bromine-2'-hydroxy-diphenyl ether which MIC are1 X 10-7g/mlStudying on the time of the suppression medicament working, we found the compounds can control and killPseudomonas. solanacearum during its logarithmic phase and stable trophophase. Along with the increasing of the compound's concentration, the effect is more obvious. Afterusing the medicament, the main factor of causing disease-extracellular polysaccharide, secreted by bacteria, is obviously reduced. Then we did the isotopic tracing marked by 3H -TDR and found this compound can evidently affect the The result of one-factor analysis of variance proved the difference of compound's concentration can do remarkable effect to the synthesis of the DNA of the bacteria. Significance test proved that the compound could reach the best control effect when its concentration is 2mg/ml. Through the photos of electron microscope, we found the compound can make the bacteria' chromatin pycnosis and destruct the formation of the cell wall then break the bacteria which is the same to the result of isotopic tracing.