Effects Of Transgenic Bt Cotton Seeds In Cultural Material On The Pests Of Pleurotus Ostreatus

Effects of transgenic Bt cotton seeds in cultural material on the pests of Pleurotus ostreatus were studied. Results were showed as followed:1. The population dynamics of edible fungi pests in the culture material composted with transgenic cotton seed and non-transgenic cotton seed were studied. The result showed that the population of Lycoriolla pleuroti and Acarid in non-transgenic cotton seed was bigger than that in transgenic cotton seed. And the inhibition to Lycoriolla pleuroti increased with concentration of cotton seed. The inhibition of non-transgenic cotton seed on Scatopse sp. was stronger than that of transgenic cotton seed, but the population of Scatopse sp. increased whit the consent of non-transgenic cotton seed. The inhibition on Megaselia sp of transgenic cotton seed was stronger than that of control when consent of cotton seed was low. While it was contrary when consent of cotton seed was high. The affect on pest was Bt protein cooperating whit cotton secondary metabolites, so there were different effects on pests.2. The results of this research indicate that the mortality of Lycoriella pleuroti larvae reared on cultures with CRI30 cottonseeds was higher than that of the larvae reared on cultures with CRI16 cottonseeds, and increased with increases in CR130 and CRI16 cottonseeds. Further, when Bt protein, gossypol, tannin and quercetrin were mixed into cultures without cottonseeds, the cultures influenced the growth of the L. pleuroti who fed on it. Of the four ingredients, the inhibition effects from gossypol and tannin were the largest. The suppression effect due to Bt protein was smallest, staying at the same level in spite of increases in Bt protein contents. Quercetrin also influenced the growth of the larvae, but the mortality level decreased with the increases in quercetrin content. Differences were also observed between the esterase isozyme patterns in the treatments and the control, indicating that cottonseeds, Bt protein, gossypol, tannin and quercetrin all affected L. pleuroti larvae.3. The effect of transgenic cottonseeds and non-transgenic cottonseeds on Lycoriella pleurati had been compared. The result shows that the mortality of Lycoriella pleurati in the cultural medium composed of transgenic cottonseed-zhong 30 is higher than that in zhong 16,with significant difference when the concentration of cottonseeds was 20 percent. And it was little higher in transgenic cottonseed SGK321 than that in SHIYUAN321 but there was no significant difference. CrylAc were detected in Lycoriella pleurati feeding on SGK321 cottonseed. Levels of CrylAc in Lycoriella pleurati declined quickly with time. The transgenic cotton-seeds restrained the growth of Lycoriella pleurati, while the non-transgenic cotton-seed zhong 36 did so. We measured the contents of cotton secondary metabolites. The content of flavonoid in zhong 30 nearly coupled that in zhong 16 and gossypol was 176.92% andtannin was 166.40%. While flavonoid -. gossypol and tannin in SGK321 was lower than that in SHIYUAN321. So the mortality of Lycoriella pleurati in zhong 30 was higher than that in zhong 16, but it in SGK321 was lower than that in SHIYUAN321.