Study On Propagation Of Several Introduced Ground Covers

This project focused on propagation of Matteuccia struthiopteris, Sedum spectabile and Hemerocallis Spp in order to establish the propagating system of each plant .The results are as follows:1.The propagation of Matteuccia struthiopteris(1)Rhizome Cuttage : Propagation of cuttage from different parts, lengths of the rhizome on different media were studied. The results showed that the combination of 2cm rhizome-cuttings and peat was the optimal method. With this method, the sprouts grew fast and average 1.90±0.64 shoots were obtained from each cutting.(2)In vitro propagatiaon: Side shoots ,originating from meristems of sectioned rhizomes, were used as explant ,and surface-sterilized with 0.025% HgCl₂ for l-3min.After 7-10d initial culture, it sprouted. A very high rate of multiplication was achieved by solid and liquid media culture. Green callus tissue produced from one young plantlet in solid medium MS+6-BA1.0mg/L+IBA0.1mg/L,changed into liquid medium 1/2MS+NAA0.1 mg/L, formed about 1736 GGB after 45d.Rhizogenesis and growth of regenerants were achieved onl/4MS+agar3g/L +sucrose7.5g/L.Transplanting medium was vermiculite, even the survival of the plantlets without root was 95.10%. 2. The Propagation of Sedum spectabile(l)Cuttage: In order to use the stocks to the utmost, the period, sort of cutting and the optimal medium ,respectively, were studied. The results indicated that at all the stages of vegetation growing, budding and early blooming , leaves could be cut for propagation, using sand as the optimal medium. During the stages of vegetation growing and budding, ,the top stem-cuttings rooted quickly with high rooting rate ,in the medium, too. Cuttings from the middle part of the stem at the stage of vegetation growing and early blooming were also good for propagation, with the sand as the medium ,however, the peat was the best of budding and post-blooming.(2) In vitro propagatiaon: Top young stem was used as explant ,and surface-sterilized with 0.1%HgCl₂ for 2min.After 7-10d initial culture in MS+2,4-D0.5mg/L+KT0.5mg/L+BA0.5 mg/L, it sprouted .A very high rate of multiplication was achieved by MS+6-BA0.1mg/L+NAA0.1mg/L,the coefficient of multiplication was22.Rhizogenesis and growth of regenerants were achieved on 1/2MS.Transplanting medium was different according to periods ,in Jan.-Feb.2v Perlite:lvVermiculite;in Mar. Vermiculite. 3. In vitro propagatiaon of Hemerocallis SppThe axillary bud stem and branching stem of young scape were used as explant, and surface-sterilized with 0.1% HgCl₂ for 3-8min.They were inoculate initially in MS+6-BA1.0mg/L+NAA0.1mg/L,MS+6-BA1.0mg/L+NAA0.01mg/L,respectiveIy.A very high rate of multiplication of H1 and H3 were all achieved by MS+6-BA1.0mg/L+NAA0.1-0.2mg/L.Rhizogenesis and growth of H1 regenerants were achieved on 1/2MS or l/2MS+NAA0.1mg/L; H3 were achieved on l/2MS+NAA0.3mg/L. Transplanting medium was lv Perlite:lvSand.