Studies On The Mechanism Of Onion Poisoning In Dogs

Six adult, clinically normal Pekingese with the body weight of 6-8kg were utilized by means of comparing the pre-experience to the post-experience in this study. They were fed cooked onions at 30g/kg body weight for 2 days. The parameters including blood routine examination, liver and kedney function, reticulocyte and Heinz body count, the activity of glucose-6-phosphate dehydrogenase (G6PD), the antioxidant system of erythrocyte, the fluidity of erythrocyte membrane, erythrocyte deformability were measured pre-feed onion and on day 1,3,5,8,12,18,24 post-feed onion. The urine was examined on the next day of phlebotomizing. Results are as follows:I.Blood and urine routine examination: By compared with pre-feed onion, the RBC, HGB and HCT decreased significantly on day 3 post-feed onion (p<0.01); MCV and MCH increased post-feed onion and increased very significantly on day 8 (p<0.01); The content of urobilinogen was increased dramatically on day 2 post-feed onion (p<0.05); and the level of Direct Bili was also increased significantly on day 1 post-feed onion (p<0.01); the specific gravity and pH value of urine changed between the normal ranges.2. Liver and kedney function: The activity of ALT and the contents of total protein, albumin only fluctuated between the normal range, and there were no differences compared with pre-feed onion (p>0.05); the activity of AST increased dramatically on day 3 post-feed onion (p<0.01); the levels of BUN and CRE fluctuated siginificantly, but also were between the normal ranges; compared with pre-feed onion the levels of Total.Bili and Direct.Bili. increased dramatically on day 1 post-feed onion (p<0.01).3. Reticulocyte and Heinz body: Compared with pre-feed onion the reticulocyte count increased sharply on day 1 post-feed onion and reached a peak on day 8 (p<0.01); the Heinz body count increased significantly on day 1 post-feed onion (p<0.01) and got to a peak on day 3.4. G6PD and the antioxidant system of erythrocyte: The G6PD's activity decreased on day 1 post-feed onion (p<0.01) and decreased the lowest on day 5, there were dramatically diferrences compared with pre-feed onion; the contents of NADPH, GSH decreased significantly on day 1 post-feed onion (p<0.01) and the levels of H₂O₂,MDA increased significantly on day 1 post-feed onion (p<0.01) compared with pre-feed onion, but the activities of GHS-px and CAT changed rulelessly.5. The fluidity of erythrocyte membrane and the erythrocyte deformability: The fluorescence polarization parameter (p) and the microviscosity (η) of the erythrocyte membrane increased dramatically on day 1 post-feed onion (p<0.01). The erythrocyte deformability (DI) appeared a downtrend since fed onion. There is a positive correlation between MDA and p,η, the correlation coefficient is 0.908 and 0.922 respectively (p<0.01); there is a negative correlation between MDA and DI, the correlation coefficient is -0.887 (p<0.05).