Studies On Construction Of TaLea₃ Gene Plant Expression Vector And Agrobacterium Tumefaciens-mediated Genetic Transformation Of Salvia Miltiorrhiza Bunge

Corps during the growth often suffers from adverse environmental stresses such as salinity, drought and low temperature. Among them, drought causes serious damage and badly influence in the yields of crops. As the farmland is limited and the population continuously increases at present, the object in plant breeding is to breed crop varieties with relatively drought tolerance. Salvia Miltiorrhiza Bunge. of Labiatae is an important traditional Chinese herbal drug, especially in curing cardiovascular disease. By genetic transformation of Salvia Miltiorrhiza Bunge. get transgenic Salvia Miltiorrhiza Bunge.of drought resisting, for enlarge the planting scope of Salvia Miltiorrhiza Bunge., have certain theoretical meaning and practice meaning. The completion of this program gets the drought resisting gene plant expression vector. We can use it for transformation plant to improve endure dry ability, and accumulate the mechanism research backlog data for the plant anti-drought.This experiment optimized Agrobacterium tumefaciens mediated genetic transformation system of Salvia Miltiorrhiza Bunge. Two vectors contained gene TaLea₃ were constructed and plant expression vector used in the genetic transformation of Salvia Miltiorrhiza Bunge. The TaLea3 gene were cloned from wheat. The transgenic plants were obtained and carried on relevant molecular biology analysis and drought resistance identification. The drought-resistance species or the breeding materials for further study were expected to obtain from the transgenic plants. At the same time these results should give useful references for application of this technology in other crops too. Main result and conclusion are the following:1. Construction of anti-drought gene plant expression vector: Using clone vector pGEM-T and plant bivalent expression vector pBIN438 as the basic component, one plant expression vector, which contained the wheat TaLea3 gene DNA, was constructed through multiple recombinations. 2 x CaMV 35S promoter drove the gene individually and the plant expression vector were used in the next experiment. Determining rank indicated that the list of TaLea3 gene is long 687 bp; this gene segment has a 636 bp...