| The dried root and rhizome of Salvia miltiorrhiza Bunge (Lamiaceae, Salvia), one of the popular traditional Chinese medicine is officially listed in Chinese Pharmacopoeia under the name Danshen. Its red skin, thick flesh and high content of active components, So DanShen cultivated in Zhongjiang Sichuan was better than other area. Zhongjiang countuy was considered as the genuine producing area of DanShen. Investigations were carried out on the changes of plant endogenous hormones and the physiological characteristics of S.miltiorrhiza under water stress, Eeffect of epibrassinolide on drought and water resistance of S. miltiorrhiza. Our objective was to elucidate the physiological responses and the changes of plant endogenous hormones of different S.miltiorrhiza under water stress. The finite element method can provide academic base for drought and Hooding resistance mechanisms, and was to take evidence for breeding S.miltiorrhiza varieties and planning of cultivation technology of resistance to drought and waterlogging. The main achievements as follows;l. Drought stress of9days with20%PEG was the optimum condition for the simulation of drought stress. In the early stage, the leaves of S.miltiorrhiza for test were relatively high in the activity of SOD. POD, CAT, whereas low in Pro. and no significant difference in MDA compared with control without25%PEG treatment. But with the time prolonging, the SOD, POD and CAT activity gradually decreased, while Pro increased, and MDA was the same as in the early stage. Under the same degree of drought stress. SOD and POD activity of Tissue big-leaf S. miltiorrhiza was highest, and that of Tall big-leaf S.miltiorrhiza was lowest; CAT activity of Tissue culture lobular S. miltiorrhiza was highest, and that of Tissue culture big-leaf S.miltiorrhiza was lowest; Wild S.miltiorrhiza of Zhongjiang county contained the most Pro; Tall big-leaf S.miltiorrhiza contained the least MDA, whereas all the other contained the same MDA.2. Waterlogging stress of5days was the optimum condition for the simulation of waterlogging stress. In the early stage, the leaves of Salvia miltiorrhiza Bunge for test were relatively high in the activity of SOD, POD, CAT, whereas low in Pro, and no significant difference in MDA compared with control. In the late stage, the SOD and CAT activity gradually decreased, while Pro increased, and MDA was the same as in the early stage. Each variety had different physiological and biochemical response to waterlogging stress. Under the flooding stress, SOD and CAT activity of Tissue big-leaf S.miltiorrhiza was highest, and that of Tissue culture lobular S.miltiorrhiza was lowest; POD activity of Dwarf big-leaf S.miltiorrhiza was highest, and that of Tissue culture lobular S.miltiorrhiza was lowest; Tetraploid lobular S.miltiorrhiza contained the most Pro and MDA, while wild S.miltiorrhiza of Zhongjiang county contained the least Pro; Tissue culture big-leaf S.miltiorrhiza, Tall big-leaf S.miltiorrhiza and Dwarf big-leaf S.miltiorrhiza contained the same MDA, lower than other three varieties.3. With the intensifying of drought stress, ABA content of leaves from six varities of S.miltiorrhiza increased in varying degree; GA3content of leaves from Dwarf big-leaf S.miltiorrhiza, Tissue culture big-leaf S.miltiorrhiza and Wild S.miltiorrhiza of Zhongjiang county showed the decreasing trend, and that of Tetraploid lobular S.miltiorrhiza, Tall big-leaf S.miltiorrhiza and Tissue culture lobular S.miltiorrhiza increased first and then decreased; IAA content of leaves from Tissue culture lobular S.miltiorrhiza, Tissue culture big-leaf S.miltiorrhiza and Tetraploid lobular S.miltiorrhiza showed the decreasing trend, and that of Tall big-leaf S.miltiorrhiza, Dwarf big-leaf S.miltiorrhiza and Wild S.miltiorrhiza of Zhongjiang county showed the increasing trend. 4. With the waterlogging time prolonging, ABA content of leaves from six varities of S.miltiorrhiza increased in varying degree, while GA3decreased; Dwarf big-leaf S.miltiorrhiza, IAA content of leaves from Tissue culture big-leaf S.miltiorrhiza and Tetraploid lobular S.miltiorrhiza had the larger range of change, which looked like W shape, and that of other three varieties gradually descended firsth, then ascended and descended lastlv.5. The activity of SOD, POD, CAT and the content of Pro was increased, while the MDA reduced with the BR in S.miltiorrhiza leaves under water stress, so the high ability of removing active oxygen was maintained in S.miltiorrhiza leaves under water stress, which reduced the accumulation of free radicals, released the damage to biomembrane system and retarded the membrane-lipid pemxidation. So the permeability of plasmic membrane diminished, cellular contents and ion leakage cut down. Therefore, BR could effectively induce the drought and waterlogging tolerance of S.miltiorrhiza.0.1mg/L of BR had the distinct after effect. |
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