| Oncidium is one of very high value arethusas. Though few of them are used as basin flowers, most of them are suitable to cutting flowers. However the propagation coefficient is not high because the whole industrialization level of raising Oncidium. is low. In the same time, the supply of many value species is comparably short, the demand is increasing, and the price is extremely high. Therefore the tissue culture of Oncidium is a key process to solve this problem. This experiment takes root stump as explant and adopts the regeneration way of bud clumps to establish a rapid technique system of micropropagation in Oncidium. The main contents focus on the following aspects, such as the choice of explants, asepsis methods, basic mediums and raising modes, the combination and density of hormone, addictions, carbon source and the density of active carbon. The Orthogonal Design is adopted to arrange experiment. Through above research, this paper tries to expand the choice scope of explants, improve the propagation coefficient and find the main factors or the suitable medium in each stage. Then filtrate the technique parameter combination, build up the superior regeneration system in Oncidium to improve the commerce production efficiency, resolve the problem that demand exceeds supply and provide the technique support. The result shows that the effective flow of asepsis method is follow : root stumps →wash down the clay by tap water →dip in the detergent water about 10min. and brush the surface dirty away →wash about one hour by tap water →put the root stumps on the extra-clean desk→dip in the 70% alcohol about 30s →wash 3 times by asepsis water →dip in the 0.2% Hgcl2 8min → wash 8 times by asepsis water→dry water→inoculate. The main factors of initition are BA and natural additions. The subordination factor is NAA. The best inducement medium is KC+BA4.0mgL-1+NAA0.4mgL-1+ coconut milk 150mlL-1+Vc0.2mgL-1+active carbon 1.0gL-1+sugar 30gL-1+agar8.0gL-1+pH5.4. The main factors of multiplication of bud clumps are the basic medium, BA, NAA, the height of seedling, cluster density, active carbon , the pH of medium, and the mode of seedling. Taking the seedling about 3cm height, cutting vertically at the bottom of seedlings and putting 4 seedlings in every 150ml bottle can obviously improve the multiplication of bud clumps. Taking active carbon 1.0～2.0 gL-1 in the medium and adjusting pH to 5.1～5.4 also can improve the multiplication of bud clumps. The subordination factor is natural addition that coconut milk 150mlL-1 or tomato juice 150mlL-1 are both good. The effect of carbon source is not obvious. The best multiplication medium is MS+BA4.0mgL-1+NAA0.4mgL-1+ coconut milk 150ml L-1 or tomato juice 150mlL-1+active carbon 1.0～2.0gL-1+Vc0.2mgL-1+active carbon 1.0gL-1+sugar 30gL-1+agar8.0gL-1. The superior medium of initiation and multipulation for PLB is MS +BA3.0mgL-1+NAA0.2～0.4mgL-1+coconut milk 150mlL-1 or apple juice 150mlL-1+Vc0.2mgL-1+active carbon 1.0gL-1+sugar 30gL-1+agar8.0gL-1+pH 5.4. The medium for promoting seedling is KC+NAA0.2 mgL-1+ apple juice 150ml L-1+Vc0.2mgL-1+active carbon 1.0g L-1+sugar 30g L-1+agar8.0g L-1+pH 5.4. The medium for rooting is KC+BA4.0mgL-1+NAA0.2mgL-1+apple juice 150mlL-1+Vc0.2mgL-1+active carbon 1.0gL-1+sugar 30gL-1+agar8.0gL-1+pH5.4. When transplanting, bark can be used in the former stage ,and when the number of new roots are enough ,the seedling should be moved into the sphagna to raise the height and bulb. The living rate can reach 100%.
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