Analysis And Classification Of Pineapple By AFLP

Pineapple, originated from South America, has become an important feature and economy fruit in tropic. However research in the breeding of new variety of pineapple has not been well developed to keep up with the pace of its industrialization in in recent years. Some important issues, such as the classification of germplasm resources, the breeding with sexual cross and early selection of important genes are all important research subjects to be addressed. The Paper introduces firstly AFLP molecular marker technique into researching germplasm identification , varieties classification and family relationship of pineapple genus plants at Guangdong Fruit Tree Research Institute, Guangdong Academy of Agricultural Sciences from July 2003 to April 2005. Which provide theoretical foundation for the evaluation, classification, utilization and selection of pineapple germplasm resources. The main research results obtained are as folio wings:1. The content of phenolic compound, polysaccharides and other secondary metabolites is high in pineapple leather leaves, which influences frequently the yield and quality of genome DNA when being extracted or being analyzed with AFLP. The result showed that the modified CTAB method found in this paper could effectively eliminate the affections of the secondary materials to extracted genome DNA from pineapple leaves, and which be suitable for AFLP analysis.2. Eight pairs of primers P-GAA/M-CTA, P-GAC/M-CTG, P-GAC/M-CTT, P-GAG/M-CAT, P-GAG/M-CTA, P-GAT/M-CAT, P-GAT/M-CTA, P-GAT/M-CTC are chosen from 64 pair of primers with AFLP analysis, and the clear polymorphic fingerprints were obtained which showed that it is feasible for using these primers in pineapple AFLP analysis. And a total of 454 sites are amplified, among which 332(73.1%) sites are polymorphic. The identification rate among 39 germplasms of pineapple genus are up to 100%.