| The larvae of Monochamus alternatus Hope feed the phloem organism and xylem of pines, which result in the death of pines through destroying the plant transporting organization and affecting the metabolism of nutrient and water. The adults spread PWN through feeding the new healthy pines. Control of Monochamus alternatus is an efficient way to cut down diffusion of PWN. Therefore, Monochamus alternatus is one of the important wood-boring pests and the key intermediary of the Pine Wood Nematode. The paper studied on a new artificial diet for Monochamus alternatus and observed the sawyer development and propagation in Zhejiang. We radiated the callow of Monochamus alternatus in 20~40Gy 60COγand searched the best radiated dosage for Monochamus alternatus eclosioned 3~5 d and the best released proportion of radiated sawyers. Moreover, the experiments tested the management effects of irradiation sterility in the forest. The results indicated that: 1. Raising artificially of Monochamus alternatus Monochamus alternatus was fed with a new artificial diet in elements and antiseptic nutrition which was added to deal crumbs and wheat embryo. Results of the vitality and the ability of propagation, which was presented in the life-history, showed that it was a successful larva diet of Monochamas alternatus. Rraising artificially, larva phase of Monochamus alternatus had eight ages. Under the condition of 25℃and 65% humidity, egg period was 5~7d, stage of pupation was 10~20d (the average time was 13.70d), the life expectancy of female and male were 36.54d and 43.14d respectively, and the rate of egg laying reached to 87.54%. 2. Searching the best radiated dosage for Monochamus alternatus The callow of Monochamus alternatus was radiated molted 3~5 ddys, in 20~160Gy 60COγand researched the radiated sawyers after copulated with the healthy ones. The indices of longevity, oviposition and egg hatching of the irradiated healthy copulated adults showed that: the irradiation made Monochamus alternatus worms sterile; the males were more sensitive to irradiation than the females; 40Gy was the best dose of the sawyer full sterility, and 20Gy was the minimum sterility dosage. 3. Researching the radiated sterile effects of Monochamus alternatus The callow of Monochamus alternatus was radiated molted 3~5 ddys, in 20~160Gy 60COγand researched the radiated sawyers after copulated with the healthy ones. The indices of longevity, oviposition and egg hatching of the irradiated healthy copulated adults showed that radiating the adults of Monochamus alternatus which molted 3~5 d shows fine control effects. The irradiation made Monochamus alternatus worms sterile; radiating the females showed better effects than treating males, the control rates of these two treats were 35.62% and 32.83% respectively. 4. Searching the best release rate of Monochamus alternatus Competition was set between the radiated sterile larva of different density and the healthy larva of fixed density, through the research on indexes of protection effects such as longevity, oviposition and egg hatching, the results showed that: the best release rate of both male and female larve of radiated sterile are 2 times of the healthy larve. 5. Testing the changes of Monochamus alternatus Physiological indices for response to the radiation ①Sperm: There was no obvious difference between the shapes of the healthy adults and treats; ②Lipase-isoenzyme: The active lipase-isoenzyme bands differ on different sawyers. The healthy and treated sawyers both showed six lipase-isoenzyme bands, in which two were consistent and three bands had obvious differences in color. E2 band of the health was lost in the treated ones, and another E2' band appeared in the treat. By comparing the pedigrees of lipase-isoenzyme of the treated ones and before, it was found out that the radiation caused the sawyers physical changes.
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