Effects Of AM Fungi On The Growth And Chemical Composition Of Bupleurum Chinense DC. Under Different Phosphor Applied Amount

Phosphor is one of the indispensable nutrition on plant growth and development. One-third to one-second soils are absent phosphor in Chinese cultivated land. Mass fraction of available phosphor of soils less than 10mg/kg are existing throughout the country. In northward area of China, soils lack of phosphor has become one of the chief limiting factors about yield increase of crops. Increasing of phosphorus fertilizer accommodating will be limited about the quantity of phosphate .Moreover chemical industry can not satisfy the phosphorus fertilizer requirement in agricultural production. Under this circumstance, it is important about finding existence of mycorrhiza and studying absorption mechanism of mycorrhiza to phosphorus. Pot experiment was conducted to study the effect of the content of phosphorus application on the infection of AM fungi and study the related effect of AM fungi on growth and chemical composition of Bupleurum chinense DC. Flowing is the main results: It's showed that AM fungi and Bupleurum chinense DC. can form symbioses efficiently. Inoculating AM fungi obviously develop mycorrhizal infection. The content of phosphorus affect the growth and development of AM fungi and formation of mycorrhiza . Symbiotic effect was the best of AM and Bupleurum chinense DC when phosphorus application was 0.3 P₂O₅g/kg soil, the infecting rate can reach 78.0%. The infection rate of inoculating Glomus mosseae and Glomus caledonium mixing was the best among three different fungi. At the suitable phosphorus levels, inoculating AM fungi can improve chlorophyll and carotennoid content in Bupleurum chinense DC leaves.The effect of AM fungi on the content of soluble protein change with application phosphorus .Except the results of 5^th-dicember ,soluble protein content of inoculating Bupleurum chinense DC was lower than non-inoculating'under the same P elvels in 0gP₂O₅/kg soil to 0.4gP₂O₅/kg soil,when the application phosphorus was 0.5gP₂O₅/kg soil, the results is controversy. The result showed that inoculating AM fungi can delay decreasing soluble protein and promote the content of soluble protein under high P level . Inoculating AM fungi can promote some chemical constituent. Experimental result showed that inoculating AM fungi can promote soluble sugar and reducing sugar content of Bupleurum chinense DC leaves and roots. Under the same P level, the content of soluble sugar and reducing sugar of the inoculating AM fungi'was higher than non-inoculating'. Under suitable P levels, inoculating AM fungi can increase some protective enzyme (CAT and POD and SOD) activity. Experimental results showed that the effect of three AM fungi on CAT and POD and SOD activity was different. Inoculating Glomus mosseae was the best on CAT activity; Inoculating Glomus mosseae was the best on POD activity; Inoculating the mixing of Glomus mosseae and Glomus caledonium was the best on promoting SOD activity. Inoculating AM fungi can increase some chemical composition of Bupleurum chinense DC. Experimental showed that saikosaponin-a and polysaccharide contents of roots and flavolodis of leaves increased with the P content increasing, when the P content was 0.3gP2O5/kg soil, the effect was the best. Inoculating three AM fungi can increase saikosaponin-a and flavolodis content. The difference of inoculating and non-inoculating Bupleurum chinense DC got obviously level(P< 0.05),but between inoculating Bupleurum chinense DC was not obviously difference. Inoculating Glomus mosseae and the mixing of Glomus mosseae and Glomus caledonium can increase saikosaponin-a and flavolodis content, but inoculating Glomus caledonium was not obvious role. Total P and total N contents of roots were higher than steams and leaves all different treatments. Experimental results showed that inoculating three AM fungi can promote total P content of roots and over ground part . Over ground part total N content of inoculating Glomus mosseae and Glomus caledonium was higher than non-inoculating'. Over ground part total N content of inoculating the mixing of Glomus mosseae and Glomus caledonium was lower than non-inoculating'. Total N content of inoculating three AM fungi was lower than non-inoculating'in roots. Inoculating AM fungi can increase root dry weight of Bupleurum chinense DC. and it should be the best combination relations between AM fungi and amount of phosphorus . Inoculating Glomus mosseae on root dry weight was the best impact under the 0.4gP2O5/kg soil level.