Bupleurum Chinense And Narrow Leaf Bupleurum And Its Phylogenetic Analysis

This article through the growing trend investigation. time of emergencenvestigation. Organization cross section structure observation. Chromosome nuclearanalysis. sozyme and RAPD analysis to discusse two kinds of Bupleurum chinensis'sbiology characteristics and the distinction method. Its result is as follows:1 The growing trend investigation and the time of emergence investigation oftwo kinds of Bupleurum indicate, The Bupleurum chinense DC's average time ofemergence is 37 days, The Bupleurum socorzonraerolium Willd is 66 days,Bupleurum socorzonraerolium Willd is later 29 days than the Bupleurum chinenseDC; Two kind of Bupleurum chinensis' high assume the"S" growth. Respectivelyare (?)北=16.02/(1+2.86e^-0.077x) and (?)狭=24.31/(1+2.84e^-0.068x); The Bupleurumchinense DC's average root is 6.35 and the Bupleurum socorzonraerolium Willd'saverage root is 3.52g. The difference is remarkable in 0.05 level.2 To the Bupleurum chinense DC and the Bupleurum socorzonraerolium Willd'sroot. stem department and leaf's department cross section structure observationindicated, The Bupleurum socorzonraerolium Willd root cross section marrowdepartment has the crystal, the Bupleurum chinense DC does not have. Throughobservation Bupleurum chinensis root cross section marrow department whether hasthe crystal, may distinguish two kinds of Bupleurum chinenses.3 The nuclear analysis of Bupleurum chinense DC and Bupleurumsocorzonraerolium Willd indicate: The Bupleurum chinense DC's chromosomenumber is 2n=18 and The Bupleurum socorzonraerolium Willd's chromosomenumber is 2n=8. The nuclear formula respectively is 2n=2sm+14m+2st and2n=4sm+4m; The chromosome relative length range of variation respectively is6.84～17.73 and 16.57～34.60, The average arm compares respectively is 1.93 and1.80.4 Use the polyacrylamide gel electrophoresis technology to picks from thedifferent local 12 Bupleurum chinensis sample's peroxide enzyme and esteraseisozyme's analysis discovery, 12 Bupleurum chinensis sample's peroxide enzymeand esterase isozyme appear 14 enzyme position spots, The peroxide enzyme has 2 common bands and the esterase has I common band.The cluster analysis resultindicated, The Bupleurum chinense DC and the Bupleurum socerzonraeroliumWilld's blood relationship relations is far, The Bupleurum chinense DC has certaindifference in planting.5 Use the RAPD technology to picks from the different local 12 Bupleurumchinensis sample blood relationship's analysis discovery, Directs the thing with 12 toexpand increases 122 bands, 106 are the polymorphism belt, Its ratio is 86.9%.Thecluster analysis result indicated, The Bupleurum chinense DC and the Bupleurumsocorzonraerolium Willd's blood relationship relations is far. The Bupleurum chinenseDC has certain difference in planting. Because the peroxide enzyme, the esteraseisozyme and RAPD analyze the obtained result to be completely same, also theperoxide enzyme isozyme band quite is clear, the dyeing drugs price is inexpensive,I suggest the next attention analysis peroxide enzyme isozyme.