The Comparison Of Effects Concerning The Crude APP And PPP On The Growth And Metabolization Of Mice

Pancreatic polypeptide (PP) is a 36-amino acid polypeptide hormone secreting from animal pancreas, PP plays important role in promoting growth, accelerating diet, modulating the level of gland and hormone. After crude avian Pancreatic polypeptide (APP) and pig Pancreatic polypeptide ( PPP) were acquired, tested and purified, the following tests on animals were done. The detail scheme is as follows, namely, 126 Kun Ming mice(4 weeks old) were randomly grouped into 9 groups and each group is 14(7 males and 7 females). Ⅰ , Ⅱ and Ⅴ, Ⅵ test groups were feed(empty stomach) With crude APP and PPP by 4μg,16μg (APP and PPP concentration is 20μg/ml and 80μg/ml respectively ) in the morning, Ⅲ, Ⅳ and Ⅶ, Ⅷ test groups were injected hypodermically crude APP and PPP by 0.2 ml, Ⅸ test group was control group.At the end of After 30 days, weighed and sampled; some function analysis and immunohistochemistry research of blood, livers and muscle were proceeded. The results showed that (1) PP can promote growth by means of enhancing enzyme activity and strengthening metabolization.(2) Both taking orally and injection can accelerate organism .growth, modify damaging organism and advance immunity and concentration of immune-related protein.(3) The difference of concentration and ways of using has differential effects on accelerating weight, the study scans the suitable dose and ways of using. (4) APP and PPP have similar function and differential effect on both accelerating growth and advancing immunity. (5) The conclusion coming from the research of immunohistochemistry and comparison primary structure between APP and PPP, it shows that the APP in mice has the same role as PP. The above results can provide some theoretical basis for the construction and function of PP and their effect on growth and metabolization-related enzyme activity.