| Multiple ovulation and embryo transfer (MOET) has the potency to increase the genetic progress in cattle. However, the wide application of MOET in cattle was restricted by the superovulation and embryo transfer level. These experiment studied superovulation of Holstein heifers and embryo transfer of recipient which come from farmer in countryside . The result was as follows:(1) In the Holstein heifers superovulation experiment, FSH (Instititute of zoology. Chinese of science) dose 7.0mg, 8.0mg and 9.0mg were used. Superovulatory responses (transferable embryo) was greater when heifers were offered 8.0mg(5.80±2.97) than when offered 7.0mg(4.83±2.23), 9.0mg (5.13±2.03). Using the FSH (Folltropin -V, produced in Canada ) dose 280mg, 320mg,360mg. Superovulation response(transferable embryo) was greater when heifers were offered 320mg(5.25 + 1.50)than when offered 280mg(4.25±2.63),360mg(5.00 ± 2.94). Using the different kinds of FSH(produced in Canada and Instititute of zoology. The numbers of transferable embryos were significantly increased (P<0.05) when heifers were offered 320 mg Folltropin-V (5.25 ± 1.38 ) compared with 8.0mg cFSH (2.67 ±0.81).(2) There were no significant difference(P>0.05) between the synchronizate oestrus of PG and CIDR for synchronizate oestrus rate (83.87% versus 65.52%), pregnance rate(52.9% versus 60%).(3) In this experiment, the recipients were random divided groups about different breeds and year, In the 1.5-3 year old , there were lower transferable recipients rate (29.59% versus 48.72%, P<0.05)and pregnance rate (20.69% versus 57.89%, P<0.01)when the Luxi cattle as the recipients than crossbreds of Simmental as the recipients . there were pregnance rate (18.18% versus 57.89%, P<0.01)when the crossbreds of Charolais as the recipients than crossbreds of Simmental as the recipients, the Luxi cattle as the recipients the transferable recipient rate(29.59% versus 43.75%, P<0.05) and pregnance rate(20.69% versus 42.86% P<0.05 ) were significantly increased when the cattle were in 1.5-3 year old than over 3 year old. the crossbreds of Charolais as the recipients pregnance rate(18.18% versus 40% P<0.05 ) were significantly increased when the cattle were in 1.5-3 year old than over 3 year old.(4) In A one-step method for direct nonsurgical transfer of frozen-thawed bovine embryos emperiment, A grade of embryo were random divided four groups: the first group, the embryo holding 5min before seeded and holding 5 min after seeded; the second group, the embryo holding 5 min before seeded and holding 10 min after seeded; the third group, the embryo holding 10 min before seeded and holding 5 min after seeded; the forth group, the embryo holding 10mim before seeded and holding 10 min after seeded, pregnance rate(66.7% versus 28.6%%, P<0.01;66.7% versus 33.3%, P<0.01; 66.7% versus 19.04% P<0.01) were significantly increased in the first group than others.(5) The recipients were random divided three groups : the first group was A grade CL, the pregnance rate was 73.33%; the second group was B grade CL, the pregnance rate was 33.33% ; the third group was C grade CL, the pregnance rate was 0 . pregnance rate(73.33% versus 33.33%%, P<0.05; 73.33% versus 0, P<0.01; 33.33% versus 0 P<0.01 ) were significantly increased in the first group than others.(6) The recipients were random divided two groups: the first group were injected P4 , the second group was control. No significant effect on pregnance rate of injected P4.(7) The recipients were random divided four group : the first group were injected VADE , the second group were injected NaSeO3) the third group were injected Vade ,+NaSeO3 , the forth were control. No significang effect on pregance rate of injected Vade > NaSeO3, VadE ,+NaSeO3.The recipients were random divided two group: the first group were feeded high level (16%SSCP <20%) CP, the second group were control(12%sSCP^16%). Transferable recipients rate(18.46% versus 53.19%, P<0.01 ) and pregnance rate(56.00% versus 16.70%%, P<0.05) were significantly decreased in the first group than control, pregnance ratewas greater when heifers were offered 2.5kg feed(66.7%) than when offered 1.5kg feed (50%).(8) Economic growth was greater when make new technological than basic technological.
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