Studies On Fertile Expression Of Male Sterility Flax In Different Ecological Conditions And Characteristic Of Cytology

Thermo-sensitivity male-sterile flax S1 and S5 which were obtained by induction of antibiotic were studied by means of different ecological site test, different sowing date test and temperature control test in 2003-2004. The relativity between fertility and temperature (photoperiod) were analyzed. Meanwhile, by the observations and photograph, the differences between two male sterile lines and fertile line were compared. In another test, techniques of microphotograph were used to observe cytology characters of abortion of male sterility lines. The main results are as follows: 1.In selective test conditions, male sterility lines could grow, blossom and maturity. But, in different ecological site and in different sowing date, male-sterile lines S1, S5 and fertile line were dissimilar at growth stage and growth period duration, the relativity between long or short of growth period duration and temperature. 2.Study on fertile expression of male sterility flax in different test condition, by analysis, intensive of illumination and photoperiod didn't had an significant effect on fertile expression of male-fertile lines; the temperature of flowering phase had an significant effect on fertile expression of male sterility. In relative low temperature, Seed-setting rate bagged of male-sterile lines were low; in relative low temperature, Seed-setting rate bagged of male-sterile lines were high. The result of every experimental site showed, at the beginning of florescence fertility were lower; after florescence, fertility of sterile lines fluctuated. By the observation, The sterile lines changed with the temperature of flowering phase regularly In temperature control condition, the fertility results of different constant temperature test were approved the significant relation between temperature and fertility of male-sterile lines. It is sterile at lower conditions and fertile at higher conditions. 3.By regression analysis between fertility and temperature and regression model, the critical temperature of sterile lines were forecast and the mean value of temperature was from 13℃　to15℃. 4.In different temperature conditions study blossoms of male sterility lines and male fertile line, the results showed: in relative low temperature condition, anthers of sterility lines S1 and S5 were yellow; anthers of fertile line are blue. In relative high temperature condition, anthers of male sterility lines and fertile line are showed the similar flower shapes. 5.Techniques of microphotograph are used to observe cytology characters of abortion of male sterility lines, and compare the differences between two male sterile lines and fertile line. Conclusion: the abortion stage of male sterility lines was from single nucleolus microspore telophase to two nucleolus microspore phase. 6.This experimental result can utilized to technical reference of seed propagation and seed production. In sum, this test was studied what fertility had relation to temperature in different ecological sites, the decisive factor making an important role is temperature , light length have a little of effect. the critical temperature for fertility transformation is 13℃-15℃. Thermo-sensitivity male-sterile lines are defined what temperature affect fertile expression of male sterility flax, and the abortion stage of male sterility lines. These research resultts are foundation which thermo-sensitivity male-sterile flax can be applied to production and study cytology.