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Effect Of Enzyme Preparations On Characteristics Of Sorghum Silage

Posted on:2006-06-20Degree:MasterType:Thesis
Country:ChinaCandidate:H P YanFull Text:PDF
GTID:2133360155953819Subject:Animal Nutrition and Feed Science
Abstract/Summary:PDF Full Text Request
Two experiments were arranged in the study. One was conducted to study the effect of different enzyme levels(cellulase and. xylanase) on characteristics of sorghum silage from storage stage and it's digstibility in fistulated dairy cows. Another was to study the effect of enzyme at a certain level on characteristics of different sorghum silages ensiled for 60 days and it' s digstibility in fistulated dairy cows.1. Effect of cellulase and xylanase at different levels on characteristics of sorghum silage ensiled for 4-90 days and digetibility in dairy cows.Silage was made from late-dough stage hybrid forage sorghum late in September, 2004. Forage sorghum had been wilted for one night before silage was made, which ensured the DM of forage was about 25-30%, then forage stuff was cut to l-2mm lenght.Five groups were arraged which included 4 treatments with different enzyme levels and 1 control without enzyme. Triplicates silos for each group were opened after 4,8, 15, 31, 60, and 90d of ensiling. Silage samples were processed and analyzed and 60d silage digstibility was determined at all enzyme levels. Treatments were arranged as follows:Group A:Cellulase and xylanase were applied at 3 ECU/g 和 0. 15 IU/gforage, respectively.Group B:Cellulase and xylanase were applied at 15 ECU/g 和 0.15 IU/gforage, respectively.Group C:Cellulase and xylanase were applied at 15 ECU/g 和 0.75 IU/gforage, respectively.Group D:Cellulase and xylanase were applied at 30 ECU/g 和 0.15 IU/gforage, respectively.Group E:Cellulase and xylanase were applied at 0 ECU/g 和 0IU/gforage, respectively.The results showed that:1). Enzymes had no significant effects on terminal (90d) pH(p>0. 05) of silages, pH of silages showed a decrease with enzyme increase. pH of treatments with enzymes declined radically early in ensiling period, especially group C, D did so(p<0. 05).2). Enzymes had no significant effects on silage ammonia level(90d) with ammonia level declined while enzymes level increasing(p>0. 05).Ammonia level of group D lowered dramatically after 30 and 60d ensiling(p<0. 05).3)The NDF,ADF contents of group D after 90d ensiling declined significantly(p<0. 05) and lactic acid level increased significantly (p<0. 05) compaired with control.4)Enzymes lowerd 24h DM, ADFand NDF digestibilities of silages after 60d ensiling(p>0.05). Enzymes lowerd 48h DM, ADFand NDF digestibilities of silages after 60d ensiling significantly(p<0.05).2. Effects of enzyme preparations on silage characteristics made from several hybrid soghum after 60d ensiling and digestibilities in dairy cows.4 treatments (sorghum hybrids) and their controls (4) were arranged in the experiment triplicatedly. Samples were got to assay their nutritive composition and digestibilities. Experiments were arranged as follow:Group B(Jincao): Cellulase and xylanase were applied at 15 ECU/g fU 0.15 IU/gforage, respectively.Group E(Control, Jincao):0Group F(Ganlu): Cellulase and xylanase were applied at 15 ECU/g $i 0.15 IU/gforage, respectively.Group G(Control, Ganlu):0Group H(Dalishi): Cellulase and xylanase were applied at 15 ECU/g #1 0.15 IU/gforage, respectively.Group I(Control, Dali shi):0...
Keywords/Search Tags:Cellulase, Hemicellulase, Sorghum, Silage, Rumen-digestibility
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