Study On Biological Effects And Mechanism Of Wheat Induced By Mixed Particle Field

The　experiment　was　designed　to　explore　the　induction　mechanism　of　simulative　aerospace　particle　on　the　earth,γ-ray-radiation　as　control,　mixed　particle　field　exploded　by　The　Beijing　Electron　Positron　Collider(BEPC),and　　was　used　to　irradiate　dry　seeds　of　Triticum　aestivum　.　In　the　range　of　the　dose　from　0　to　560GY,　experimental　result　show　that　mixed　particle　field　has　significant　mutagenic　effect　on　the　M1　generation,　decreased　seed　germinating　ability,　seed　germinating　rate　has　fall;　increased　chromosomal　aberration.　The　frequency　of　chromosomal　aberration　on　the　root　tip　cells　and　biological　damage　on　the　seedling,　there　are　significantly　dose-effect.There　are　higher　　chromosome　aberration　frequency　and　more　chromosome　aberration　types　induced　by　mixed　p article　field　　than　γ-ray-radiation.M1　generation　seed　grown　in　experimental　field　show　different　biological　damage,　such　as　emaciated　plant,　even　dead.　An　analysis　of　SSR　polymorphism　show　mixed　particle　field　have　made　wheat　DNA　serious　damage,　various　damage　type　can　be　seen　through　band　on　denature　gel.　The　majority　of　SSR　polymorphism　of　treatment　has　less　bands　than　control.　B　genome　has　more　stronger　genetic　polymorphism　than　A　and　D　genome,　and　become　'hot'　after　irradiation.　There　are　11　pair　primers,　most　of　them　distributed　in　B　genome,　shown　good　polymorphism　to　distinguish　different　mutgents　and　dose　among　48　pair　primers.　In　M2　genernation　group,　there　are　many　mutations,　including　spike　mutation,　awn　mutation,　etc.　Mixed　particle　field　induced　more　mutation　types　and　higher　mutation　frequency　thanγ-ray-radiation,　and　dose　during　200-300GY　is　suitable　for　mixed　particle　field　to　irradiate　wheat.ZhonGyou9507　has　more　mutation　types　and　mutation　frequency　than　ZhonGyuan9.The　comparison　betweenγ-ray-radiation　and　mixed　particle　field　show　that　mixed　particle　field　have　stronger　aberrations　and　biological　damage.　The　different　variety　show　different　response　ability　to　irradiation,　zhongyou　9507　show　more　sensitivity　than　zhongyuan　9　induced　by　γ-ray-radiation　and　commingle　particle　field.