Effects Of Rapeseed Meal On The Immune Function Of Carassius Auratus Gibelio

The study was conducted on Carassius auratus gibelio, the effects of rapeseed meal to C. auratus gibelio were detected by the non-specific immunity and the ability of immune response.In the experiment of study on effects of rapeseed meal on the non-specific immunity, soybean meal (SM) and fish meal (FM) were used as basic protein source in the control group (â… ), with 33.43% (â…¡), 66.86% (â…¢) of double-low rapeseed meal (DLRM) and 34.97% (â…£), 69.94% (â…¤) of common rapeseed meal (CRM) to replace the protein of SM 50%, 100% respectively in the control group, the another group was the commercial feed. The effects of rapeseed meal on the non-specific immunity were detected by the phagocytic and bactericidal activity of the blood leucocytes and the phagocytes in the head kidney, lysozyme activity in serum, the content of serum complement C3 and C4, number of the intestinal intraepithelial lymphocytes (iIEL). The main results were summarized as follows: With the increasing of the rapeseed meal levels and the prolong of the breeding, the effects of rapeseed meal on the non-specific immunity became obviously. The phagocytic and bactericidal activity of the blood leucocytes and the phagocytes in the head kidney, lysozyme activity in serum, the content of serum complement C3 , C4 and the relative number of the intestinal intraepithelial lymphocytes (ilEL) were depressed tendency. In the result, the test group V (comprising 69.94% CRM) changed the earliest and biggest, the flowing group was the test group III (comprising 66.86% DLRM), in the 56th day, the immune indexes of the test groups (III, V) were all significant differences with the control group (P<0.05). The phagocytic and bactericidal activity of the blood leucocytes and the phagocytes in the head kidney, lysozyme activity in serum, the content of serum complement C3 and C4 of the test group IV (comprising 34.97% DLRM) were also depressed tendency, but no significant differences with the control group. The test groups (III, V) had no significant differences each other.In the experiment of study on effects of rapeseed meal on the ability of immune response, soybean meal (SM) and fish meal (FM) were used as basic protein source in the control groups (A, a), with 33.43% (B, b), 66.86% (C, c) of double-low rapeseed meal (DLRM) and 34.97% (D, d), 69.94% (E, e) of common rapeseed meal (CRM) to replace the protein of SM 50%, 100% respectively in the control groups, the another groups (F, f) were the commercial feed. In the test groups, groups (A-F) were immunized C. auratus gibelio and groups (a-f) were not immunized. The main results were summarized as follows: The immunocompetence of groups (C, c; D, d; E, e) were decreased all long. From the beginning of 21th day after immunization, the all immune indexes of the testgroups (C, c and E, e) were significantly different with the control groups (A, a) (P<0.05). From the beginning of 35th day after immunization, the several immune indexes of the test groups (D, d) were significantly different with the control groups (A, a) (P<0.05). After challenged with live Aeromonas hydrophila, mortality of the test groups (E, e and C, c) were significantly higher than the control groups (A, a). The 6 test groups (A-F) of immunized C. auratus gibelio compared with the 6 control groups (a-f) of not immunized C. auratus gibelio correspondingly, the phagocytic activity of the blood leucocytes and the phagocytes in the head kidney, lysozyme activity in serum, the content of serum complement C3 and C4, agglutinating antibody titers were all significantly higher (P<0.05), the bactericidal activity of the blood leucocytes and the phagocytes in the head kidney was little higher though no significant differences.