The Study On Molecular Markers Linked To Powdery Mildew Resistance Genes By SSR In Wheat Cultivar SARKA And Yumai34

The disease for wheat make it has yield lose and quality low. Powdery mildew, caused by Erysiphe graminis f.sp tritici, is a destructive disease of common wheat throughout the world. Breeding and popularizing resistance cultivars has been proved the most effective, economical and environmentally safe method to suppress the pathogen, broad-spectrum powdery mildew resistance. Therefore the exploitation of mildew resistant genes, which including it's marking, location and molecular-marker aided selection, would benefit breeding the effective powdery mildew resistant cultivars.In this study,the resistant gene of Powdery mildew of wheat varity SARKA and yumai34 was analysis by using SSR technique.The results are showed just as follows:1.The 300 F₂ individuals and their SARKA(resistant mother) and henong822 (susceptible father) carrying unknowing gene were inoculated with mildew strain at seedling stage to assess their resistant ability. Genetic analysis suggested that the resistance of SARKA was controlled by a pair of single recessive gene.2.The 300 F₂ individuals and their yumai34(resistant mother) and zhengyou5 (susceptible father) carrying unknowing gene were inoculated with mildew strain at seedling stage to assess their resistant ability. The results showed that the resistance of yumai34 was controlled by a pair of single recessive gene too.3.Ten of 300 DNA samples extracted from the F₂ population were pooled into two separate groups for bulked segregant analysis (BSA).By SSR analysis with 4 pairs of primers located on 1A chromosome in 300 individuals of SARKA X henong822,4A chromosome in 300 individuals of yumai34×zhengyou5, F₂ segregation population and their resistant and susceptible parents. We screened a molecular marker Xgwm99 and Xgwm357 that linked to the SARKA resistant gene, the genetic distance among marker Xgwm99, Xgwm357 and the resistance gene was calculated as 34.7cM and 29.9cM. We also get the result that Xgwm397 that linked to the yumai34 resistant gene, the genetic distance among marker Xgwm397 and the resistance gene was calculated as 42.4cM.