Research On Molecular Marker Technology For Peanut Varieties Identification

Germination percentage, authenticity and purity of peanut varieties are important items for measurement in peanut seed testing. The present study was conducted mainly in 3 aspects: optimization in peanut seed germination and viability test, biochemical and DNA molecular markers for peanut variety identification.1 Seed germination percentage and viability difference of different maturation seeds were very great.The seed germination percentage and the dry weight of seedling of ill maturation seed fell clearly andthe viability was lower.2 . The seed germination percentage and seed viability on different bud bed were different. The testresults on sand bed were better than on paper bed. At the same time, the test results were different whileseeds were placed on sand bed on different position, and on different paper bed (BP/TP).3 Nine peanut cultivars fell into 3 groups according to their seed storage protein banding pattern, wherepresence/absence, darkness and migration rate of each band were analyzed; however, only a limitednumber of peanut varieties may be identified.4 . The technology of SRAP SSR AFLP molecular marker in peanut variety identification were studied using 10 peanut cultivars mainly from Shandong. Of the 51 SRAP primer pairs tested, 2 produced monomorphic bands only, and forty-nine totally produced 1,087 bands, among which 503 were polymorphic, the varieties could be grouped into 4 categories based on cluster analysis of the SRAP fingerprinting data. 62 SSR/STS primer pairs were found to produce polymorphic bands, and 291 out of 427 (68.1%) were polymorphic. Nine primer pairs differentiated each of the individual the peanut cultivars. 8 AFLP selective amplification primer pairs were used to discriminate these varieties, the results showed that utilization any one of the primer pair failed to identify each of the cultivars, while primer pair combinations could differentiate each cultivar.5 Taken into account its high discrimination power, robustness, reliability and feasibility, as demonstrated in this study, the SSR/STS marker system turned out to be the best technical option in the context for peanut variety identification.