Optimization Of Regenerated System And Anatomical Study Of Tissue Structure In Cymbidium Goeringii Cv. Luyun With Verge Line Pattern Leaves

Cymbidium with verge line pattern leaves is the total appellation of five Oriental Orchids varieties with verge line pattern, which are Cym.goerymgii, Cym.emsifolium, Cym.faberi, Cym.Sinense, and Cym.KanranMakino in Orchidaceae Cymbidium. Cymbidium with verge line pattern leaves is the aberrance variety .The lamina has art phenomena such as yellow, white or stripe verge line pattern. Its genes are brought aberrance because of environment stimulate such as illumination and lightning strike. This cymbidium is more valuable than other orchids, as its leaf has beautiful color.But its propagating coefficient is very low by traditional method of crown division. And art phenomena on the lamina aren't inherited steadily on account of levity on environments and planting condition. So many rare cymbidium breeds are lost.Tissue culture of Oriental Orchids has some work over such as Cym Sinense.But there isn't study on the tissue culture of Cymbidium with verge line pattern leaves yet. The ideal rapid multiplication system of Cymbidium goeringii cv. Luyun with verge line pattern leaves by protocorm like-body was established. Then origin of proliferation and differentiation of rhizomes was studied. Effects of plant growth regulators on rhizome ' s multiplication and differentiation were studied. Furthermore, causation of verge line pattern leaves was discussed ordinarily by observation on cell ultrastructure of verge line pattern leaves.The main results are summarized as follows:1 .Optimization of regenerated system in Cymbidium goeringii cv. Luyun with verge line pattern leaves(1) Effects of cytokinins KT and BA are favorable to the multiplication of rhizomes, but rhizomes were vitrifiable easily when KT concentration over 1.0 mg L~-1;the number of rhizomes increased with the increasing of auxin concentration (0-3 mg L~-1), thereinto IBA was more effective than NAA.(2) Rhizomes on the basic culture medium B5 proliferate rapidly, but they began tobrown after 50 days cultured. Growth of rhizomes on 1/2 MS solid medium was better than on B5.S0 B5 is favor of short-term fast multiplication, and 1/2 MS is propitious to long subculture and preservation. Study showed: It was better for rhizomes' multiplication and growth in culture converted of 1/2 MS and B5 than in any.(3) 1/2MS supplemented with BA 1.0 mg-L"1 and IBA 1.0 mg-L'1 was successful for rhizome's differentiation, Number of buds differentiation after 80 days unto 36.9%.(4) Optimal subculture time is 80 days to accelerate differentiation of rhizomes. The reason is dedifferentiation accelerated via restraining vegetative growth of rhizomes.(5) Dasheen paste added to the culture medium was better to the multiplication of rhizomes than coconut milk and banana mud .The rhizomes were well by the way, which had broken off with fingers and thumb, each conglomeration with 3-5 rhizomes.2. Rise and genesis of rhizome's multiplication and bud's differentiationApical differentiating tissue divides continuously and prolongates to become to the rhizome;internode cortex cells of rhizome divides continuously to form adnation differentia tissue, then develop directly to bud or branch of rhizome. Clustered rhizomes with multi differentia tissues subdifferentiate buds.3. Plant structure anatomise of tube-plantlet and study of lamina cells' ultrastructure(1) Verge line pattern on the leaf of Cymbidium goeringii cv.'Luyun' test-tube plantlets was clear, and younger leaf 's verge line was so more than old one that it could be stably inherited.(2) Lamina surfaces of Cymbidium goeringii with verge line pattern leaves are covered with thick cutin.Epidermis cells array tightly and there are many vascular bundles clinging epidermises.The mesophyll is not differentiated into spongy and palisade tissues, and clearance is smaller. Vascular bundles of leaf with parallel venation are collateral bundles.(3) Vascular boundless in pseudobulb are scattered in ground tissue. In cross section view, from outer to inner part, root includes velamen, cortex, and vascular cylinder. Pseudobulb and root all have the function of storing and keeping water. It's typical shade-demanding and drought-enduring plant. So water management is key to the young plant surviving in nursing management of cymbidium.(4) Cells'ultrastructures were viewed of one lamina's art segment (meaning: yellow segment) and normal green segment. Pilot study indicated the primary causation of etiolating : (T).amount of chloroplast decreases;(2). chloroplast ateliosis, some swell, mostly is former-body of chloroplast -proplastid, few thylakoids, just grana.