Research In The Affection Of Quercetin, Curcumin And The Other Six Effections On Motsoh Larva' PPO

The Motsoh (Anoplophora glabripennis) is a notorious Trunk borer, which eats the woodand settles in wood. Recently, part of gardens has fallen deeply into the victim of the Motsh.In 1998, the U.S.A government declared a bill that it would forbid imports the woodenpacking and matting material from China to prevent the hazard of a kind of pest-Trunk borer.It stated that once the pest got prevalent in U.S.A, it would have brought a direct loss of 138billion dollar in the journalism as well as eco-environment. In 2002, The Wood Eaten PestPreminar between U.S.A and China stated that the motsh have been a severer threat to thewood industry, especially to the "Three North" protecting wood belt.The harm of Trunk borer has received more and more attention .The thesis Of biologicalpesticide chosen PPO as target are still scare. The research with Psoralen, Magnolol, Osthole,Chlorogenic acid, Andrographolide, Curcumin, Quercetin , coffee acid phytochemistricalmaterial with different biological activity as the effection was conducted to validate theanti-Trunk borer technique.The Trunk borer is been selected as object to study. The result revealed that when thedeposits disposed by 40 per cent saturated ammonium sulfate solution and then purified, thetotal reclaim rate of enzyme is up to 30.08%, increased more than 4 folds comparing nothingtreatment condition . The test on crude enzyme of the Trunk borer larva discovered that , thePPO in the period of pupa is transparently higher than growing phase.Selecting the PH=6.5,T=37℃ and the optimal reaction time setting as 2min to havestudied the influence of eight substrates which including Psoralen, Magnolol, Osthole,Chlorogenic acid, Andrographolide, Curcumin, Quercetin , coffee acid to the PPO of Trunkborer. The results indicated that the strongest inhibition among the eight substrates is theQuercetin solute by methanol, and then the Osthole, Chlorogenic acid, Curcumin, coffee acid.But the PPO in all the eight substrates is minor than trial group. However, Osthole appears tobe the strongest inhibition in the test of PPO activity of worm, if the effects solutes by thephosphate-methanol buffer solutes .And following are Osthole, Chlorogenic acid,Andrographolide, coffee acid, Magnolo. And the PPO in all the eight substrates is minor thantrial group.Two groups are divided to study the enzyme inhibition mechanism analysis arefocus on the Quercetin and Curcumin. One is measuring the enzyme change with differentenzyme volume of 0.02,0.05,0.1,0.15,0.2 following the different effect concentrations of0.1,0.3,0.5,0.7,0.95μg/mL but keep the identical substrate concentration. The other group isthe measuring the enzyme change with same enzyme volume however different substrateconcentrations of0.001,0.005,0.01,0.05,0.1mol/L and following the different effectconcentration of 0.1,0.3,0.5,0.7,0.95μg/mL. The inhibition dynamics of Quercetin andCurcumin expressed by Bi-reciprocal diagram indicated that the change of Vmax almosteffected nothing to Km, Therefore , such a conclusion can be drawn that the these twoeffects is non-competition inhibitor, and the inhabitation constant are 1.886μg/mL and1.4549μg/mL respectly.Two groups are divided to study the enzyme inhibition mechanismanalysis are focus on the Quercetin and Curcumin. One is measuring the enzyme changewith different enzyme volume of 0.02,0.05,0.1,0.15,0.2 following the different effectconcentrations of 0.1,0.3,0.5,0.7,0.95μg/mL but keep the identical substrate concentration.The other group is the measuring the enzyme change with same enzyme volume howeverdifferent substrate concentrations of0.001,0.005,0.01,0.05,0.1mol/L and following thedifferent effect concentration of 0.1,0.3,0.5,0.7,0.95μg/mL. The inhibition dynamics ofQuercetin and Curcumin expressed by Bi-reciprocal diagram indicated that the change ofVmax almost effected nothing to Km, Therefore , such a conclusion can be drawn that thethese two effects is non-competition inhibitor, and the inhabitation constant are 1.886μg/mLand 1.4549μg/mL respectly. The thesis about pest-inhabitation by applying Magnolol andOsthole has proposed long time for years, however, in pest field were scarce. The inhabitationmechanism of Magnolol and Osthole to PPO of the Motsoh worm appears to benon-competition . Thereinto, the Magnolol set phosphate as buffer solution can reflect thereaction which catalyzes L dopamine. However, a further analysis about the effect of Ostholeneed judge the influence from carbinol to enzemy, under the condition that Osthole wassoluted by carbinol.