RAPD Markers And Sequence Analysis Of Specific Fragment Linked To Dominant Genomic Sterile Gene Of Flax

A total 252 arbitrary 10-mer primers were used to screen markers linked to the dominant male genomic sterile and fertile gene in flax. The optimum conditions of RAPD reaction were studied. It was found that three primers of S135,S62and G06, which could stably amplify specific DNA fragments and the relevant percentages are 71.4%,75.0% and 83.3%, respectively. S62 and S135, respectively, could produce RAPD markers correlated with dominant male genomic sterile gene, named S62-500 and S135-350. G06 could produce RAPD marker correlated with dominant male genomic fertile gene, named G06-650.The cloned S62-500,S135-350 and G06-650 specific fragments were sequenced and analyzed the sequence with BLAST. The results of sequence analysis showed that S62-500 correlates closely with the genomic sterile gene of flax, S135-350 has the correlation to the genomic sterile gene of flax and fertility restoration gene, G06-650 may be correlative to the gene of dominant earliness of flax.