Rapid Propagation Protocol In The Culture Of Cymbidium Kanran Makino And In Vitro Flowering

Using Cymbidium kanran Makino as material, we studied a rapid propagation for culturing Cymbidium kanran Makino and flowering in vitro.The results showed that the optimal medium for seed germination was 1/2 B₅ containing 0.6 mg L^-1 6-BA, 0.2 mg L^-1 NAA and 0.05 % AC, in which the rate of seed germination was 28.5 % after about 120 d. Multiplication of rhizome was optimum in B₅ containing 0.6 mg L^-1 6-BA, 1.2 mg L^-1 NAA with a 1.77 growth rate. B₅ containing 1.0 mg L^-1 6-BA, 0.2 mg L^-1 NAA was fit for differentiation of rhizome. To induction of lateral bud, B₅ (1/10 N, 5 P ) containing 6-BA 3.0 mg L^-1 was found to be suitable, bud multiplication coefficient being 5.9. B₅ containing 0.50 mg L^-1 TDZ, 0.25 mg L^-1 NAA was most effective for induction of PLBs, in which rate of PLBs was up to 98.3 %. For multipliction of PLBs, the best medium was B₅ containing 1.0 mg L^-1 S-3307, 0.2 mg L^-1 NAA and 3.5 % sucrose with about a 9.4 multiplication rate. For differentiation of PLBs it was B₅ containing 0.75 mg L^-1 S-3307,1.0 mg L^-16-BA and 0.4 mg L^-1 NAA, in which the differentiation rate was up to 87.8 %. B₅ (1/10 N, 5 P) containing 6-BA 3.0 mg L^-1 was appropriate for induction of clustered shoots from PLBs, the multiplication coefficient being 5.6. For rooting it was best on 1/2 B₅ supplemented with 0.20 mg L^-1 NAA and 0.05 % AC, in which the rooting rate reached 100 %. As for transplantation, the best substrate was the pearlite, sawdust and grass-ash in proportion 1:1:1, whith about a 97.4 % survival rate after 150 d.At the same time, the results showed that floral bud induction rate was 58.33 %...