Studies On Submerged Culture Technics For Cordycepin Production By Medicinal Fugus Cordyceps Militaris

Cordyceps militaris , a caterpillar-shaped Chinese traditional medicinal entomopathogenic fungi, which was used as functional food and medicine because of its various activities. This fungi produces an important bioactive compound, cordycepin(3'-Deoxyadenosine). How to enhance the cordycepin production is a bottleneck for commercial application of fungi fermentation technology.The objective of the present study was to investigation the optimal culture conditions for mycelial biomass and cordycepin production by Cordyceps militaris (CM8) in submerged culture.The one-factor-at-a-time method was used to investigate the effects of medium components (i.e. carbon, nitrogen, mineral sources and growth factor) and environmental factors (i.e. initial pH and temperature) on mycelial growth and cordycepin production. The optimal initial pH and temperature for mycelial growth were 6.0 and 20℃, and for cordycepin production were 5.0 and 26℃, respectively. Among these medium components, sucrose, peptone, MgSO₄, K₂HPO₄, NAA were the most suitable carbon, nitrogen, mineral sources and growth factor, respectively. Subsequently, the concentration of these medium components was optimized using the orthogonal layout method. The optimal concentration for mycelial growth were 40g 1^-1 sucrose, 25g l^-1 peptone, 0. 5g l^-1 MgSO₄·7H₂O, 1.5g l^-1 K₂HPO₄ 2.0mg l^-1 NAA, and 40 g l^-1sucrose, 15 g l^-1peptone, 0. 5 g l^-1MgSO₄·7H₂O, 0. 5 gl^-1 K₂HPO₄, 4.0mg l^-1NAA for cordycepin production, respectively.This optimization strategy in shake flask culture leads to a mycelial...