Research On The Cultures Of Cordyceps Militaris And Its Physiological Active Components

Cordyceps militaris, as a substitute of winter-worm summer-grass (C. sinensis), is an important species in Chinese traditional medicine, from which cordycepin and cordycepic polysaccharide are the two main biological components. In this thesis, it is reported that the influence of light and temperature on C. militaris in solid culture, the effect of the nutrients (carbon source and nitrogen source) and temperature on the mycelium biomass and cordycepic polysaccharide content in liquid culture, and the influence of cordycepic polysaccharide on the longevity of fruit fly were studied.In the solid culture process, the suitable temperatures for the development of C. militaris are between 17-25 ℃, the optimum temperature for the growth of fruitbody is 22 ℃ The mycelia of C. militaris can grow well in dark, while 150LX or more of light is needed in the differentiation of the fruitbodies. And even the light intensity up to 6000 LX, the fruitbodies could grow well. However, natural light possibly inhibit the growth of fruitbodies. There is a positive relationship between the cordycepin content of C. militaris fruitbodies and light intensity and temperature. During the growth of the fruitbody of C. militaris, dark processing can increase the weight and diameter of the fruitbody.In liquid culture, the mycelium of C. militaris can use different concentrations of carbon source and nitrogen source, and the mycelium grew best in the liquid media with 6% of carbon source and 1% of nitrogen source. The most appropriate ratio of the carbon source/nitrogen source is 6/1 for the growth of C. militaris mycelium. The optimum temperature for the growth of mycelium is 25 ℃ which is also the optimum temperature for the mycelium to synthesize polysaccharide.The yield of extracellular polysaccharide reached the highest in the optimum temperature for the growth of mycelium, any other temperatures did not promote accumulation of the extracellular polysaccharide. It is referred that the yield of extracellular polysaccharide be correlated with the biomass of mycelium.