Study On The Tissue Culture And Plant Regeneration Of Wheatgrass

The study in this paper using the immature embryos of wheatgrass(Agropyron Gaertn.—A.mongolicum Keng, A.desertorum cv.'Nordan', A.cristatum×A.desertorum cv.'Mengnong') to affect callus induction by age of immature embryos, genotypes, mediums and 4℃processing;Studying the feasible subculture medium of A.desertorum cv.'Nordan'and A.cristatum×A.desertorum cv.'Mengnong';studying the effect of wheatgrass callus differentiation by mediums, genotypes, hormone and AC. The results were as follows:1)The feasible medium of A.mongolicum Keng:The callus inducing medium: MS+CH(500mg/L)+2,4-D(2.0mg/L)+6-BA(0.05mg/L)Subculture medium: MS+CH(500mg/L)+2,4-D(1.0mg/L)Differentiation medium: MN+ CH(500mg/L)+NAA(0.5mg/L)+6-BA(4.0mg/L)2)The feasible medium of A.desertorum cv.'Nordan':The callus inducing medium: MS+CH(500mg/L)+2,4-D(2.0mg/L)+ABA(0.7mg/L)Subculture medium: MS+CH(500mg/L)+2,4-D(1.0mg/L) +6-BA(0.5mg/L)Differentiation medium: MN+ CH(500mg/L)+NAA(0.5mg/L)+6-BA(4.0mg/L)3)The feasible medium of A.cristatum×A.desertorum cv.'Mengnong':The callus inducing medium: MS+CH(500mg/L)+2,4-D(2.0mg/L)+ABA(0.3mg/L)Subculture medium: MS+CH(500mg/L)+2,4-D(1.0mg/L) +6-BA(1.0mg/L)Differentiation medium: MN+ CH(500mg/L)+NAA(0.5mg/L)+6-BA(4.0mg/L)+AC(3.0mg/L)4)The feasible rooting and strong medium of the three varieties :Rooting medium: 1/2MS+ CH(500mg/L)+NAA(0.5mg/L);Strong medium: 1/2MS+ CH(500mg/L)The other study in this paper is repress the precocious germination of A.mongolicum Keng's immature embryos by cane sugar, CH and ABA. The results were as follows:1)The immature embryos can repress the precocious germination in the medium: MS+ cane sugar(5%)+ CH(500mg/L);2)The immature embryos can repress the precocious germination in the medium with 0.2mg/LABA or 0.4mg/LABA.