The Study On Genetic Diversity Of The Roe Deer (Capreolus Capreolus Bedfordi) In The Heilongjiang Province, China Base On Faecal DNA Extract Technique

The roe deer is an important economic animal in China. Up to now, pocaching roe deer and their habitat fragmentation or losses have reduced population size and geographical range. Therefore, understanding the morphological and molecular variation of different geographic population of roe deer is important for us designing conservation strategies. The 32 faecal samples from three differences geographic regions: the Great Khingan Mountains, the northern part of the Lesser Khingan Mountains and the Wandashan Mountains in Heilongjiang Province, Norheastern China, were sequenced and population analysis. The results show: (1) In this study, the length of the D-loop of mtDNA genome from 32 samples was 460bp. 50 sites were polymorphic. It was indicated that the general genetic diversity population was of higher level as shown of by following parameters: the mean value of haplotype diversity (h) was 0.9880;the mean value of nucleotide diversity (p) was 0.022 60. (2) 27 haplotypes were identified from 32 sequences. (3) Among the roe deer population of three geographical units, the geographic region, the Great Khingan Mountains, have higher numbers of base differences, higher nucleotide diversity and higher average number of nucleotide differences. (4) No obvious phylogenetic structure among haplotypes was found for samples of different origins. (5) The freezing-method is better than 90% ethanol preserved and oven-dried stored at extracting faecel DNA. The freezing-method is simple in collection and preservation, saving-time and economic. As a result, it may be applied to faecal sample of all ungulate animals living cold area in winter.Based on these results, we suggest some conservation and management of roe deer population must be taken to effect. In the Great Khingan Mountains, the roe deer population of was relatively abundant genetic diversity. Therefore, in term of protecting gene diversity of this population, we propose classifying it as the unit of high priority protection in the future...