| In this paper, the super-microstructure,bioassay and biological control of Clostera anastomosis L.GV were discussed systematically by the methods of electron microscope, paraffin section. DNA of CaGV was extracted from genome and granulin gene was produced by PCR. Nucleotide and amino acid sequence of granulin gene were analyzed and the secondary structure was predicted, which provided the basis for the function of granulin gene in infecting larvae.The results of this study were as follow:(1) The shape of CaGV was oval or ovate, and some were anomalous under the scanning electron microscope.The average size was 428.54×237.96nm. The virus particle was composed of virus pole, intima and utricle under transmission electron microscope and the average size was 223.44×58.51nm.(2) The result of bioassay indicated that the regression equation between the logarithm of the virus concentration and the mortality was y=1.946+0.558x. The LC50 was 2.97×105PIB·L-1.The LT50 for the virus concentration of 1.58×105 , 1.58×l06,1.58×l07, 1.58×l08,1.58×109 PIB·mL-1 were 8.55d, 6.89d, 5.9d, 4.65d, and 4.08d, respectively, shorting gradually with the concentration increasing.(3) CaGV formed in the midgut epithelia cell under the electron microscope by observing the paraffin section. Nucleolus became bigger and the midgut epithelia cell distorted seriously. But the malpighian tube and peritrophic membrane were no harm.(4) A fragment of 498 bp was produced by PCR from CaGV DNA. The fragment was sequenced after being cloned into pGEM-T Easy. Compared with CacGV, PsGVA25-6, CpGVA11-2,AoGVS45,CmGVA11-1,HcGVA5-l, the identity of nucleotide and amino acid sequence with CacGV was highest. In the analysis with basic property of protein, there...
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