Effect Of Several Pesticides On The Activities Of Detoxifying Enzymes And Protective Enzymes In Clostera Anastomosis

In this paper, we assayed the toxicity of six insecticides to Clostera anastomosis by leaf film method. By treating vitro enzymes and the live insects, we studied the effect of beta-cyfluthrin、lambda-cyhalothrin、diflubenzuron、riflumuron、imidacloprid and abamectin on the activities of detoxifying enzymes and protective enzymes in the 3rd instar and the pupa of Clostera anastomosis. The result will provide a theoretical basis for exploring the mechanism of insecticide, scientific application of insecticide and improvement of control efficacy.1. Their LD50 of six insecticides are different, their toxicity order is as the following: lambda-cyhalothrin> beta-cyfluthrin> abamectin> imidacloprid> diflubenzuron> triflumuron.2 The result of enzyme activities showed that, all the six insecticides had different effect on the in vitro detoxifying enzymes and protective enzymes in two different instars of Clostera anastomosis. The activities of in vitro detoxifying enzymes in the 3rd instar and the pupa were highly inhibited by antibiotics insecticide (avermectin) and pyrethroid insecticides (beta-cyfluthrin and lambda-cyhalothrin); The activities of in vitro protective enzymes in the 3rd instar were weakly inhibited by neonicotinoid insecticide (imidacloprid), which highly inhibited the activities of in vitro detoxifying enzymes in the pupa; The activities of in vitro protective enzymes in the 3rd instar were highly inhibited by pyrethroid insecticides (beta-cyfluthrin and lambda-cyhalothrin), antibiotics insecticide (avermectin) and carbamate insecticides (diflubenzuron). Except beta-cyfluthrin, the other five insecticides had good inhibition effect on in vitro protective enzymes in the pupa. Overall, the in vitro detoxifying enzymes and protective enzymes in the 3rd instar of Clostera anastomosis are more sensitive to six insecticides than that in the pupa.3. On one hand, the activities of in vivo detoxifying enzymes and protective enzymes in the 3rd instar of Clostera anastomosis were not only related to the type and the sensitivity of enzymes, but also to the type of insecticides, action mechanism and action time of insecticides. Beta-cyfluthrin and lambda-cyhalothrin showed inhibition effect after the first activation effect on the activities of in vivo CarE; triflumuron inhibit firstly and then activate the activities of in vivo CarE; diflubenzuron showed activation -inhibition-activation effect, imidacloprid and abamectin showed high inhibition effect on the activities of in vivo CarE. As to the activities of in vivo GST, beta-cyfluthrin had inhibition-activation-inhibition effect; lambda-cyhalothrin showed activation effect; the other insecticides activated firstly and then inhibite the activities of in vivo GST. As to the activities of in vivo AChE, beta-cyfluthrin and lambda-cyhalothrin showed activation first and then inhibition effect; diflubenzuron and triflumuron had activation effect; imidacloprid showed activation-inhibition-activation effect, Whereas avermectin had inhibition-activation-inhibition effect.The result of the activities of in vivo SOD showed that, beta-cyfluthrin and avermectin showed activation first and then inhibition effect; triflumuron had inhibition first and then activation effect; diflubenzuron and imidacloprid showed activation effect, whereas lambda-cyhalothrin had inhibition-activation-inhibition effect. As to the activities of in vivo CAT, beta-cyfluthrin showed activation effect; during early treatment cyhalothrin showed activation effect with low concentration and inhibition effect with high concentration, and it showed activation effect during late treatment. Diflubenzuron had activation first and then inhibition effect on the activities of in vivo CAT, whereas imidacloprid and avermectin mainly showed inhibition effect.