Studies On Chemical Constituents

Inula cappa DC., which belongs to Compositae family, is a kind of important folk medicine. The root and the whole herb of I. cappa can be used for treatment of eliminating phlegm, antiasthmatic, promoting blood circulation to restore menstrual flow, injurues and so on. I. cappa in Guizhou folk is widely used conventional medicines by many ethnic minorities. I. cappa was a major component of a formula“Ya-Jiao-Ha-Dun-San”, which is a kind of Dai tranditional madicine. It can be used to treat rheum, laryngotracheitis, irrrgular menstrual periods, abdominal pain and so on. Besides, accroding to "The research and development of guizhou miao yao" records, I. cappa was an important Miao tranditional medicine, which were usually collected in summer and autumn, and mainly used to treat cold fever, sore throat and rheumatism sore.In order to further study on efficacy material base of I. cappa DC., find some new biological activity of compounds. the aerial parts of Inula cappa were isolated and puriflied by various chromatographic methods. 22 chemical comstituents were identified by the spectroscopic date analysis(NMR, MS, et al): they were identified as nine flavonoids: luteolin(1), apigenin(2), chrysoeriol(3), artemetin(4),2′,5-dihydroxy-3,6,7,4′,5′-pentamethoxyflavone(5), chrysosplenol C(6), apigenin-5-O-β-D-glucopyranoside(7), luteolin-3-methylether-4′-O-β-D- glucopyranoside(8),luteolin-4′-O-β-D-glucopyranoside(9), four triterpene: darma- 20,24-dien- 3β-Oacetate(10), darma-20,24-dien-3β-ol(11), epirfiedelanol(12), friedelin(13), three coumarin: scopoletin(14), ioscopoletin(15), scopolin(16), stigmasta-5,22-dien-3β-O-7-one(17), stigmasterol(18), palmitic acid(19), linoleic acid(20), linoleic acid methyl ester(21),(E)-9,12,13-thridroxyoet- adee-10-enoie acid(22). Compounds 5 is a new natural product, compound 3~9, 15, 17, 21, 22 were isolated from this genus for the first time.In the study, we focused on the biological activities of flavonoids compounds(compounds 1~9) which have been isolated from I. cappa DC. 2-fold dilution method was applied to measure the reduced bacterial activity of the flavanoids of I. cappaagainst Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus subtilis. The results showed that compound 3 had medium inhibitory effect against E. coli(the MIC was 12.5 ? g/mL), and compound 6 had weak inhibitory effect against B. subtilis(the MIC was 25 ?g/mL). Select N. glutinosa to anti-TMV activities using a screening method of leaf spot, The results showed that compounds1, 3, 5, 6 and 8 also showed inhibitory effect against the TMV virus(inhibit rates were34.8%, 43.6%, 32.9%, 37.9% and 41.8% respectively). The protect activity against TMV virus indicated that compounds 2, 6 and 7 had a strong inhibitory effect on the TMV virus(inhibit rates were 55.8%, 70.9% and 76.1% respectively), which significantly showed higher inhibition effect than the positive control building ningnanmycin(inhibition rate was 54.2%). In addition, this thesis also test the activity against tumor by method of MTT in vitro. The results showed that compound 6 had a moderate inhibitory growth activity against tumor include hel, k562, dp17, cb7 and mda in vitro, the MICs were 35.47, 43.03, 109.57, 95.92 and 141.65 μM respectively.The test of anti-inflammatory activity of compounds is in-process.