| Goal:This topic treats uarthritis’s security and the curative effect through the research fast-acting uarthritis spirit pellet, applies the fast-acting uarthritis spirit pellet for clinical on to provide the reliable experiment basis well.Methods:120 mice, preoccupy 20 (to be half and half female and male), divides five groups, does endures the quantity experiment most greatly, extracts endures the quantity multiple most greatly. Next does the sterilization analgesia experiment:The mouse hot plate experiment screens qualified female mouse 30, the heavy repetition measurement its threshold of feeling value, takes two time normal threshold of feeling averages The value, before taking this mouse to give the medicine, the threshold of feeling value. Along with the fuselage 4 groups, each group of 10 are the physiological saline group, the masculine comparison medicine group (colchicine), the fast-acting uarthritis spirit pellet height monitoring team, fills the stomach separately, fills the stomach volume is 0.4ml/, above each group for the medicine even 1/day, gives the medicine continuously on 3rd, After Moci for medicine 1.5h and 3h measure the threshold of feeling value separately; The mouse turns the body experiment to take male healthy mouse several 40, the body weight (20±2) g, along with fuselage 4 groups. Physiological saline group 10, fill the stomach 1/day, and so on volume physiological saline 0.4ml/, normal diet. For medicine group 10, fills the stomach 1/day, fast-acting uarthritis spirit pellet 0.4ml/, normal diet. Masculine control group 10, fill the stomach 1/day, colchicine 0.4ml/only (0.005mg/ml,0.1mg/kg), normal diet. Each group fills the stomach to the medicine 2 days, end time for medicine 1 hour later, injects 0.6% acetic acid solution according to the 0.1ml/10g body weight abdominal cavity, observes and records various mice to appear in 15min turns the body number of times; Mouse ear swelling method chooses mouse 30, divides into stochastically for the medicine group, the masculine control group and the physiological saline group, fills the clothing fast-acting uarthritis spirit pellet, the colchicine and the physiological saline separately,1/d, continuously 3 d. End time After for medicine 1.5 h, spreads evenly the xylene benzene around the mouse right ear both sides,40μl/, after 1h, escapes zhui the execution the mouse, along the auricle base wire shears next two ears, overcomes the round ear piece separately with 9 mm punching bears in the identical spot, the torsion balance weighing quantity, compared with gives the medicine group, the masculine control group and the blank control group swelling rate difference.Results:The fast-acting uarthritis spirit pellet mixture in asks to endure the quantity most greatly in the anxious poisonous experiment, in has endures the quantity multiple which five group of uarthritis pellet solution different density obtains is most greatly 240 times (already when is bigger than 100 times far), has not had the animal death situation, also has not seen the diarrhea symptom. In the sterilization pain-relieving experimental study, compares an uarthritis friability with the physiological saline group to suppress the xylene benzene The result mouse auricle swelling and the reduced acetic acid result turns when the body number of times has statistics significance (p>0.05)。A low dose uarthritis friability and the colchicine group compare the non-statistics significance (p>0.05), But the high monitoring team has statistics significance with it comparison (p<0.05); Compare an uarthritis friability with the physiological saline group and the colchicine group when the extension hot plate result mouse’s ache reaction time has statistics significance (p<0.05), And in each experiment the uarthritis pellet high, between the low monitoring team has certain quantity effect increasing relations.Conclusion:In the mouse experiment, the fast-acting uarthritis spirit pellet and the western medicine quite have not seen the obvious poisonous side effect, may confirm the clinical practice safe reliable; Can obviously suppress the xylene result mouse auricle swelling; Can obviously lengthen the hot plate result mouse’s ache reaction time and the reduced acetic acid result turns the body number of times, has the very strong sterilization analgesia function.
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