Study On Triterpenoids And Their Anti - Inflammatory Immune Activity In

Radix Sanguisorbae is the dried root of Sanguisorba officinalis L.or S. officinalis L.var. longifolia (Bertol.) Yu et Li (Rosaceae). It’s widely distributed in the northern districts of China. It’s cold in nature, bitter and sour in taste. Its root has hemostatic, analgesic and astringent properties, and it has the efficiency of subsidence of swelling and evacuation of pus, removing toxic materials and treatment of sore. It has been used as a herbal drug for the treatment of burns, scalds, internal hemorrhage, enteritis and dermatogic disease.Previous investigations have shown that the roots of S. officinalis mainly contain tannins, phenolic acids, triterpenoids, flavonoids, anthraquinones, cyanophoric glycosides and sterols.It has also been reported that this crude drug has exhibited many pharmacological activities, including haemostasis, anti-inflammatory, antibiosis, anti-tumor, and promoting wound healing.In this study, chemical constituents of the roots of S. officinalis from Jilin province and its pharmacological activity were investigated.70% ethanol extracts were chromatographed on macroreticular resin column, polyamide column and silica gel column, combined with Sephadex LH-20 and ODS column chromatography to give 17 compounds. Their structures were elucidated by physico-chemical properties and spectral analysis. They were identified as 1β-hydroxyeuscaphic acid (2)、euscaphic acid (1)、arjunic acid (3)、tormentic acid (4)、rosamultic acid (5)、haptadienic acid (6)、pomolic acid (7)、ursolic acid (8)、ziyu-glycosideⅡ(9)、ziyu-glycosideⅢ(10)、3β-[(α-L-arabinopyranosyl) oxy]-urs-12,18-dien-28-oic acidβ-D-glucopyranosyl ester (11)、ziyu glycoside I (12)、kaji-ichigoside F1 (13)、2α,3β,19α-trihydroxyolean-12-en-28-oic acid-28-β-D-glucopyranosyl ester (14)、2α,3β,19α-trihydroxyurs-12-en-28-oic acid-28-β-D-glucopyranosyl ester (15)、niga-ichigoside (16)、3β-[(α-L-arabinopyranosyl)oxy]-29-hydroxyolean-12-en-28-oic acid-β-D-gl ucopyranosyl ester (17). Compound 3、5、6 and 17 were isolated from this genus for the first time.With the method of polyamide column removing tannins, the total triterpenes of Sanguisorba officinalis L. (SOTT) were riched for the first time.The anti-inflammatory and analgesic activity of the total triterpenes of S. officinalis(SOTT) were investigated by acetic-acid-induced body twist method and celiac capillary permeability in rats method, which reduced the frequencies of body twist induced by acetic acid and decreased the celiac capillary permeability. The inhibition ratio is 79.6% and 50.0% respectively.Furthermore, carbon particle clearance test was used to evaluate the effect of the total triterpenes of S. officinalis (SOTT) on cyclophosphamide model mice, which showed SOTT could increase the non-specific immune function of hypoimmunity mice.