The Effect Of Cellulase And Different Chemical Reagents On Rose Seed Germination

Using rose seed as study target, this experiment tried to explain and evaluate the influences on germination, including ABA and soluble sugar content after the application of cellulose, sulfuric acid and GA. And further explore the correlation of ABA/soluble sugar content and seed germination which indicates:Either cellulose or cellulose mixed with GA application on rose can greatly enhances its seed germination rate, and above all cellulose treatment has more prominent effect on seed germination than sulfuric acid and GA which proves itself to be the most ideal choice of seed pre-treatment for high germination rate.The experiment also finds that seeds after multiple treatment shows high seed germination rate than after single treatment; which proves by the fact that seed after GA/sulfuric acid alternative treatment shows highest germination rate followed by that after either GA or sulfuric acid with the lower germination rate; Although there is accumulation effect observed after GA/sulfuric acid alternative treatment, little effect is observed after GA/cellulose treatment which can be explained by rose rigid seed shell that is insensitive to exogenous hormones.Lower ABA content is observed in seeds after cellulose treatment than the other single treatments. And we found ABA content is negative correlated with seed germination rate and thus we can judge whether seed is dormancy condition by testing its ABA content in early stages.The experiment also finds soluble sugar contents variation in seeds after different treatments and there is positive correlation between soluble sugar content in seeds and corresponding germination rate. The phenomenon can be explained by that seed with higher soluble sugar contents are normally in higher metabolism in which condition plant can provide energy and nutrition for germination, so ABA content in seed can be as index of its dormancy condition and reflection of seed metabolism.