Studies On Application Of Composite Microorganisms Groups Used For Composting Of Cow Dung

Aerobic cellulolytic microorganisms are separated from cow feces compost under 50℃. Aspergillus WQ are screened from the microorganisms. The composition Vis attained after comparing composite enzyme activity of each composition. The composition is compared with Aspergillus WQ by being added in Cow feces compost. The results indicate that the effect of the composition is better than that of Aspergillus WQ. After comparing with EM in cow feces compost , it is showed that the ability of utilizing the materials of microorganisms composition V is stronger than the ability of EM. After EM and the composition are added in cow feces compost, it is showed that EM can promote the aerobic microorganism groups to utilize the materials. There are three parts in the dissertation:1. Aspergillus WQ is selected to explore the factors which affect producing enzymes. The best carbon source is crystallitic cellulose+straw powder, the best nitrogen source is peptone+yeast abstract cream, the best growing temperature is 35℃and the best initial pH is 5.5. WQ can produce the largest quantity of enzymes after being cultivated eight days. The best reaction temperature of cellulose enzymes is 60℃.2. From the microorganisms that are screened from the cow feces compost, two bacteria, four actinomycetes and one mildew are screened. The seven microorganisms are combined with P-decomposing microorganism (Bacillus megaterium), K-decomposing microorganism (Bacillus mucilaginosus) and thermophilic cellulolytic microorganism (Thermophilic sporotrichum). After being added in cow feces compost, temperature, liquid-water content, substance changes, seed germination index and composite enzyme activity are determined during the fermentation. The ability of utilizing materials of compound microorganisms composition V is stronger than that of the single microorganism (Aspergillus WQ) fermentation.3. Compound microorganisms groups are compared with EM during cow feces fermentation. The results indicate that the ability of utilizing materials of compound microorganisms is stronger than that of EM. In order to improve the quality of the fermentation, EM and compound microorganisms groups are added in cow feces compost. The results of the temperature, liquid-water content, substance changes, seed germination index and composite enzyme activity during the fermentation show that EM can stabilize the temperature of fermentation and promote aerobic microorganisms to increase the seed germination index and decompose cellulose.