| In this experiment,30 wild Eremochloa ophiuroides germplasm resources were collected from Hunan province as research material. The genetic diversity of these materials had been studied from morphological and molecular level. The main research fruits wents as follows:1,Using the shape target to carries the heredity multiplicity analysis of 30 Hunan wild Eremochloa ophiuroides germplasm resources indicated:the variation of 30 germplasm resources appeared in 12 characters, in which root system depth, the leaf area, length of leaves-hair, the stem color and the leaf color, the variation all achieve above 20%.2,The correlation analysis on shape indicated that, the height of leaf layer altitude with the color of the leaves and other 7 observation character, except the width of leaves, the node diameter of erect stem, the root system depth, outside the stem color, assumes extremely remarkable is being connected or the inverse correlation, the value scope separately between -0.791～0.803 and-0.772～0.793. The length of leaves and other 7 observation character, except the blade width, the root system depth, outside the stem color, assumes extremely remarkable is being connected or the inverse correlation, the r value scope betwee -0.889～0.846.3,The shape cluster analysis result indicated that, the morphology cluster analysis for 30 Hunan wild Eremochloa ophiuroides germplasm resources result to indicate that, the material to be possible to divide into three shape types, namely the popular type, high large-scale and lives short.4,According to supplies to the material in the Changsha area growth green time, screened initially has suited 3 for trying the materialin Changsha uses in the lawn cultivation.5,In this experiment, based on the previous studies, a set of simple, rapid and accurate technique has been explored for Eremochloa ophiuroides DNA extraction and purification, and a best experimental system for the molecular markers of Eremochloa ophiuroides has been established.6,In this experiment, the Hunan wild Eremochloa ophiuroides germplasm resources has a rich hereditary change. Altogether examines to 126 may analyze the banding, in which many condition banding 123, accounts for 97.62%;In single share material level I=0.1922, H=0.3155; In community level I=0.0878, H=0.1302; Nei'heredity multiplicity analysis indicated, between 12 communities has had certain degree heredit differentiation(GST=0.5434).The lowest heredity multiple levels appear in the Hengyang, Loidi, Shaoyang vacation thrifty grass community.But the highest heredity multiple levels appear in the Yongzhou vacation thrifty grass community.7,Nei's heredity distance between the 30 Hunan wild Eremochloa ophiuroides germplasm resources community's range of variation is 0.0274～0.1942. Obtain using the UPGMA, law construction 12 community's heredity relations cluster charts, is away from 0.12 in Euclidean space to be possible to divide into 12 communities 6 kinds.The division class group and the geographic distribution has the obvious relations, in which Huaihua vacation thrifty grass community and the Jishou vacation thrifty grass community's relationship is recent, first gathers in the same place;The Shaoyang vacation thrifty grass community and the Hengyang vacation thrifty grass community heredity is far away from each other.8,Carries on the cluster analysis result to 30 Hunan wild Eremochloa ophiuroides germplasm resources to indicate that, when similarity factor 0.780 for threshold value, may divide into the material eight big kinds. Inherits the similarity factor division according to ISSR the class group and geographic distribution existence certain relations.This is consistent with the principal components analysis result.9,This experiment has promulgated the heredity multiplicity of 30 Hunan wild Eremochloa ophiuroides germplasm resources. The synthesis above findings may obtain, the 30 Hunan wild Eremochloa ophiuroides germplasm resources is rich in the morphology and the DNA member mark heredity multiplicity.
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