Isolation And Identification Of Chicken Salmonella And Surveillance Of Drug Resistance

Salmonellosis is a common zoonotic disease. It could not only cause serious economic losses in animal production, but also a serious threat to human health. Application of antibiotics has played a huge role in the controlling and prevention of salmonellosis in humans and livestock, but with a wide range continuous and inappropriate use of antibiotics, Salmonella resistance to antimicrobial agents has become increasingly common. 246 suspected disease of chicken feed (which is 215 21-day-old chicks, 31 were for the 15 weeks above the age of adult chickens) were isolated and identified from May 2008 to March 2010 in Tai'an City Shandong Province. In this study, by biochemical tests and serological tests, they were identified as Salmonella enteritidis (S.enteritidis) 48 strains of Salmonella Newport (S.newport) 5 strains of Salmonella typhimurium (S.typhimurium) 40 and Salmonella pullorum (S.pullorum) 153 strains. the 7 monitoring and of Salmonella were analysed by Susceptibility testing from poultry antibiotic sensitivity 42, 12 of 246 isolates and 6 of antibiotics commonly used quinolone resistance monitoring and analysis. The results showed that all isolates were sensitive to amikacin and the cephalosporin Qusum; 167 pairs showed different degrees of resistance of streptomycin, kanamycin, gentamicin, ampicillin, tetracycline, chloramphenicol, florfenicol, cephalosporin Cefotaxime, Cephalothin, cephradine, in which the highest rate of drug is streptomycin, 46.5%, followed by tetracycline (29.7%), and the remaining 79 as sensitive strains. 246 isolates, there are 52 pairs showed mild patience of nalidixic acid, ciprofloxacin, enrofloxacin, sarafloxacin, lomefloxacin, levofloxacin, and the remaining strains were sensitive strains. The trend that the emergence of drug resistance have enhanced, which may be related to Salmonella gene mutation itself or be concerned with the misuse of antibiotics. The 7 isolates doing Susceptibility testing were done pathogenic animal regression test. Results showed that mortality rate (74%) of 7 strains of pathogenic strains (98%) were high. Thus, Salmonella is highly pathogenic around Shandong Province, and preached common antibiotic resistance in different degrees, which susceptibility testing results will guide future clinical veterinary medicine extremely instructive.