| Cephalotaxus oliveri Mast.is an ancient relict plant, its leaves, sticks, seeds, roots of plants can extract a variety of alkali, for the treatment of leukemia and lymphosarcoma, such as a certain degree of efficacy, is the material used at the same time and an excellent landscape tree species used. Cephalotaxus oliveri with the development and utilization of its potential value discovery, Cephalotaxus oliveri of the rapid propagation of more and more shows the importance of it. So far, however, Cephalotaxus oliveri of the tissue culture at home and abroad to carry out very little, but did not promote the use in production. In this study, the use of plant cell culture technology, respectively, Cephalotaxus oliveri callus induction, callus tissue culture system and the suspension cell culture system and its homoharringtonine control metabolism studies.(1) Hunan three Cephalotaxus (Cephalotaxaceae, Cephalotaxus oliveri, Cephalotaxus) leaves homoharringtonine for the content of high and low:Cephalotaxus oliveri> Cephalotaxus> General Cephalotaxus(2)Callus induction is easy for Cephalotaxus oliveri. Callus can appear in every callus-inducing medium. The optimal medium for callus induction is:MS++2.0 mg·L-1 NAA+0.5 mg·L-1 6-BA+1.0mg·L-1 2,4-D+0.5 mg·L-1 KT.The optimal explains for callus induction are the leaf's base that grow in spring (from March to May), the ratio of callus inducing reach 100%.(3)Based with the medium for callus induction, the experiments research the effects of some factor to the multiplication of callus, including basic-medium, sucrose, inoculate volume, pH value and plant hormone. The optimal medium and the culture condition is: MS+2.0 mg·L-1 NAA+0.5 mg·L-1 6-BA+1.0mg·L-1 2,4-D+0.5 mg·L-1 KT, and appended 30 g·L-1 sucrose, pH 5.8,inoculate volume 2.0g·4.0mL-1.Researching the effects of culture condition and natural nutritious materials to the metabolism of honoharringtonine, appended 0.1 g·L-1,peptone can't accelerate the yield of harringtonine, but it can accelerate the growth of callus, the callus' rate of increment reach556.44%; When 0.1 g·L-1,hydrolysis casein has been append, callus' rate of increment reached330.62% and the concentration of homoharringtonine reached 16.8μg·g-1(4)In this experiment calculated the effects of culture condition to cell growth, including inoculate volume, rotational speed, sucrose, pH and basic-medium, the results were as fellow:cell grew fast when concentration of sucrose under 30 g·L-1,rotational speed reach 80r·min-1; The research of the original pH value of medium shows the biomass of suspension cell get the fastest growth at pH5.8; Higher biomass accumulation were observed in higher inoculate volume, when cell inoculated in medium that composed of MS++2.0 mg·L-1 NAA+0.5 mg·L-1 6-BA+1.0mg·L-1 2,4-D+0.5 mg·L-1 KT and 30 g·L-1 sucrose, the rate of increment reach 852.93%.(5) The research of the original pH value of medium shows the concentration of harringtonine reacted the highest at pH 6.2 though the growth was lower. The yield of homoharringtonine can be accelerated when precursors has been replenished in medium, the concentration of harringtonine (8.5μg·g-1) increase 77%.(6) Cephalotaxus oliveri solid culture and liquid culture calli homoharringtonine detection:comparison of Cephalotaxus oliveri solid callus culture and Cephalotaxus oliveri callus suspension culture in homoharringtonine content. Quantitative analysis by HPLC and found the latter homoharringtonine alkali content was significantly higher than the former. Key Words:Cephalotaxus oliveri Mast.callus culture, suspension cell culture,homoharringtonine, secondary metabolism...
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